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EC number: 930-010-9
CAS number: 461432-25-7
Table 1 Final test – Overview of the results
Mean respiration rate
% Inhibition of the respiration
(mg O2/L h)
(mg O2/g h)¹
7.6 - 8.2
7.7 – 7.8
7.5 – 7.8
7.6 – 7.8
7.5 – 7.7
7.8 – 7.8
¹) The amount of suspended solids in the final test mixture was
Table 2 Final test – Respiration rate and inhibition reference item
Respiration rates (mg O2/L.h)
Respiration rates (mg O2/g.h)1
The amount of suspended solids in the final test mixture was 1.5 g/L
This study was performed to assess the influence of the test item on the
respiration rate of activated sludge after a contact time of 3 hours.
The study procedures were based on OECD guideline No. 209 and performed
under GLP conditions.
Because the test item was not sufficiently soluble to allow the
preparation of a 10 g/L stock solution in water, it was mixed with
Milli-RO water before adding synthetic medium, sludge and the remaining
Milli-RO water was added. During 3 hour exposure period, optimal contact
between the test item and test medium was ensured by applying continuous
aeration and stirring. Oxygen consumption was recorded after 3 hours for
approximately 10 minutes.
A combined range finding/limit test was performed with loading rates of
10 , 100 and 1000 mg/L. Based on the results from this test, a final
test was conducted using loading rates of 10, 32, 100, 320 and 1000
mg/L. Five replicates per loading rate and six replicates per untreated
control were tested.
No statistically significant inibition of respiration rate of the sludge
was recorded at any of the tested loading rates in the final test.
The batch of activated sludge was tested for sensitivity with the
reference item 3,5-dichlorophenol, and showed normal sensitivity. The
study met the acceptability criteria prescribed by the study plan and
was considered valid. In the final test, the temperature was below the
range prescribed by the study plan (20 ± 2°C) during the exposure phase,
i.e. 17-21°C. However, because the validity criteria for the controls
and reference item were met, this deviation was considered to have no
effect on the study.
The test item was shown to be not toxic to waste water bacteria
(activated sludge) at or below a loading rate of 1000 mg/L (NOELR).
ELR50 could not be determined (> 1000 mg/L).
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