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EC number: 930-010-9
CAS number: 461432-25-7
bovine corneal opacity and permeability assay uses an opacity
measurement and a permeability measurement to determine a final in vitro
score. The opacity measurement is determined by the change in opacity of
each cornea by subtracting the calculated pre-treatment opacity reading
from the final opacity reading. The corrected opacity value of each
cornea was calculated by subtracting the average change in opacity of
the negative control corneas from that of the treated corneas. The mean
opacity value of each treatment group was calculated by averaging the
corrected opacity value of the treated corneas from each treatment
condition. The permeability measurements: the corrected OD 490 is
determined by subtracting the negative controls mean OD 490 from each of
the treated cornea OD 490 value. The mean OD 490 of each treatment group
was calculated by averaging the corrected OD 490 values. The formula
used is: in vitro score = mean opacity value + ( 15 x mean OD 490
Value). The in vitro score is then compared to a classification system
established based on studies with a wide range of test materials. The
classification provides a good initial guide to interpret these in vitro
data: an in vitro score from 0 to 25 is mild irritant, from 25.1 to 55
is a moderate irritant and 55.1 and above is a severe irritant.
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