Registration Dossier

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Additional information

Short-term studies

The relevant results from the available short-term studies with DOPA-Glycinate are briefly summarised in the following tables.

 

Acute toxicity to fish (Oncorhynchus mykiss):

Guideline/test method

Endpoint/type of test

Exposure

Results [µg a.i./L]

Remarks

Design

Duration

LC0

LC50

LC100

 OECD 203 (1992)

Mortality

Flow-through

96 h

84.9

207.4

n.d.

Measured conc.

 

 

Acute toxicity to aquatic invertebrates (Daphnia magna):

Guideline/test method

Endpoint/type of test

Exposure

Results
[µg a.i./L]

Remarks

Design

Duration

EC0

EC50

EC100

OECD 202 (2004)

Immobility

Semi-static

48 h

10.9

33.3

n.d.

Measured conc.

 

 

Growth inhibition in algae (Pseudokirchneriella subcapitata):

Guideline/ Test method

Endpoint/ Type of test

Exposure

Results
[µg a.i./L]

Remarks

Design

Duration

NOErC

EbC50 (1)

ErC50 (2)

OECD 201 (2006)

Growth inhibition

Static

72 h

9.55

19.5

23.7

Measured conc.

 

1) calculated from the area under the growth curve;

2) calculated from the growth rate

 

Inhibition of microbial activity (aquatic)

Guideline/ Test method

Species/ Inoculum

Endpoint/ Type of test

Exposure

Results [mg a.s./L]

Remarks

Design

Duration

EC20

EC50

EC80

OECD 209 (1984)

Activated sludge from municipal STP

Respiration inhibition

Static

3 h

11

22

44

In terms of active ingredient

 

With LC/EC50 values clearly below 1 mg/L, DOPA-Glycinate is considered as highly toxic to fish, algae and aquatic invertebrates.

Based on the acute toxicity tests in aquatic organisms, DOPA-Glycinate requires classification as hazard category Acute 1 (M factor: 10) in accordance with CLP Regulation (EC) No 1272/2008.

 

Long-term studies

The relevant results from the available long-term studies with DOPA-Glycinate are briefly summarised in the following tables.

 

Chronic toxicity to fish (Oncorhynchus mykiss).

Guideline/ Test method

Endpoint/ Type of test

Exposure

Results [µg a.i./L]

Remarks

Design

Duration

NOEC

EC50

EC10

OECD 215 (2000)

Mortality, fish weights, fish lengths, and pseudo-specific growth rate

Flow-through

28 d

≥ 52.3

n.d.

≥ 52.3

Measured conc.

 

 

Chronic toxicity to aquatic invertebrates (Daphnia magna).

Guideline/ Test method

Endpoint/ Type of test

Exposure

Results [µg a.i./L]

Remarks

Design

Duration

NOEC

EC50

EC10

OECD 211 (1998)

Reproduction

Semi-static

21 d

2.3

10.6

3.1

Measured conc.

 

 

By comparison of the NOEC or EC10 values, Daphnia magna is the most sensitive species with respect to chronic effects, based on cumulative offspring per Daphnia and intrinsic rate of increase. Oncorhynchus mykiss, by contrast, appears to be relatively insensitive, with no observed effects on any test parameter up to the highest tested concentration of 52.3 μg a.i./L. The growth rate of unicellular algae was inhibited at a test concentration of more than 9.55 µg a.i./L (NOEC=9.55 µg/L).

Based on the chronic toxicity tests in aquatic organisms, DOPA-Glycinate requires classification as hazard category Chronic 1 (M factor: 1) in accordance with CLP Regulation (EC) No 1272/2008.