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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Description of key information

21 d EC10 = 3.1 µg a.i./L; Daphnia magna, OECD guideline 211, GLP

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater invertebrates:
3.1 µg/L

Additional information

The influence of DOPA-Glycinate (20% a.i., as manufactured) on the reproduction of Daphnia magna was investigated. A 21-day semi-static exposure to the test item at nominal concentrations of 0.92, 2.30, 5.75, 14.40, and 36.00 µg a.i./L, with daily renewal of the test solutions was conducted according to OECD guideline 211. Untreated control replicates were run in parallel. Each treatment group consisted of 10 replicates with one daphnid each (individual exposure). Effects on growth (adult length at test termination) and reproductive performance were investigated. Samples of fresh and aged test solutions were analysed for test item concentrations.

Since the nominal concentrations varied by more than 20 %, the biological effects were re-evaluated based on mean measured concentrations. Due to a decrease of test item concentration during the renewal period, the time weighted mean values (TWM) for each treatment level were calculated. The respective TWM were 0.78, 2.28, 2.39, 11.35, and 27.48 µg a.i./L (85.3, 99.2, 41.5, 78.8, and 76.3 % of nominal).

All effect concentrations are given as time weighted mean concentrations:

The 21 d NOEC was 2.3 µg a.i./L (cumulative offspring per Daphnia, intrinsic rate of increase), the EC10 was 3.1 µg a.i/L (parental survival).

 

Concentration

Parental survival

Growth (length on day 21)

Age at first brood

Cumulative offspring per female

Intrinsic rate of increase

EC50
(95% CL)

10.6
(6.5–17.1)

n.d.
(n.d.)

> 27.5
(n.d.)

24.6
(11.3–>27.5)

> 27.5
(n.d.)

EC20
(95% CL)

4.7
(2.8–8.2)

n.d.
(n.d.)

> 27.5
(n.d.)

6.8
(0–14.0)

11.5
(7.4–16.2)

EC10
(95% CL)

3.1
(1.6–6.0)

n.d.
(n.d.)

24.2
(n.d.)

3.4
(n.d.–8.5)

4.4
(1.7–7.0)

NOEC

2.4

≥ 27.5

≥ 27.5

2.3

2.3