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Short-term toxicity to aquatic invertebrates

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Description of key information

48 h EC50 = 33.30 µg a.s./L (95% CL 26.05–42.57 µg a.i./L); Daphnia magna, OECD guideline 202, GLP

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
33.3 µg/L

Additional information

The influence of DOPA-Glycinate on immobilisation of Daphnia magna was investigated according to OECD Guideline 202 (2004). For this, the daphnids were placed in water containing the test item in nominal concentrations of 59.87, 101.77, 173.01, 294.10, and 500.00 µg test item per litre, representing 11.97, 20.35, 34.60, 58.82, and 100.00 µg active substance per litre. The test was conducted under semi-static conditions with renewal of test media after 24 h. Effects on immobilisation were determined after 24 and 48 hours.

Test substance concentrations were determined by LC-MS/MS. Total active substance concentrations were extrapolated from the mean measured concentrations of four representative lead components.

Actual test concentrations (time weighted means) were determined to be 3.29, 6.45, 10.87, 26.98, and 74.29 μg a.i./L (27.5 %, 31.7 %, 31.4 %, 45.9, and 74.3 % of nominal).

No significantly increased mortality (NOEC) was detected when compared to the control up to mean measured concentrations of 10.87 µg/L. The EC50 was estimated at 33.30 µg a.i./L (measured).

Thus, the recalculated effect values for immobilization at test end (based on time-weighted mean measured concentrations) are

NOEC = 10.87 µg a.i./L

LOEC = 26.98 µg a.i./L

EC10 (95% CL) = 15.86 µg a.i./L (11.21–22.45)

EC20 (95% CL) = 20.48 µg a.i./L (15.36–27.30)

EC50(95% CL) = 33.30 µg a.i./L (26.05–42.57)