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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

96 h LC50 = 207.4 µg a.i./L (CL: 157.5–273.2 µg a.i./L), Oncorhynchus mykiss, OECD Guideline 203 / EU method C.1, GLP

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
207.4 µg/L

Additional information

The influence of DOPA-Glycinate (20% a.i.) on acute toxicity to rainbow trout (Oncorhynchus mykiss) was investigated in accordance with the OECD Guideline 203 and EU method C.1 (92/69/EEC).

The fish were placed in water containing the test item at nominal concentrations of 93.5, 188, 375, 750, 1500, 3000, and 6000 μg test item per litre, representing 18.8, 37.5, 75, 150, 300, 600, and 1200 μg a.i./L. The test was conducted under flow-through conditions for 96 hours. Effects on survival were determined after 24, 48, 72, and 96 hours. Samples of test solutions were taken at test start, after 48 hours and at test end.

In test media the mean measured concentrations of the test item were in the range of 43 % to 57 % of nominal, independent of the concentration. For the evaluation of the effect concentrations the means of the measured four highest concentrations were used. At nominal concentrations up to and including 84.9 μg a.i./L, neither mortality nor abnormal condition or behavior was observed. At higher concentrations, there was a clear concentration and time-effect dependency, starting with 30 % mortality at 136.3 μg a.i./L on day four and ending with 100 % mortality at 672.5 μg a.i./L on the first day. Clinical signs of intoxication were apparent only in surviving or moribund fish at concentrations causing partial or total mortality.

The 48 h LC50 was 351.0 µg a.i./L, the 96 h LC50 was 207.4 µg a.i./L (CL: 157.5–273.2 µg a.i./L).

Since the validity criteria are fulfilled - except maintenance of test concentrations within 20 % initial, which is however addressed by consideration of measured concentrations - the study is considered to be valid.