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Short-term toxicity to fish

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Description of key information

LC50 was estimated to >0.28 and <0.73 mg/l. NOEC was estimated to be 0.28 mg/l.
Due to the low water solubility of TS-ED 532, the effect observed was considered to be a physical effect rather than a toxic effect, due to partitioning of TS-ED 532 onto the gills of the fish. The NOEC was several magnitudes higher than the estimated solubility concentration of 2 x E-4 mg/l, and TS-ED 532 was thus considered to be nontoxic within its solubility range. Based on these data, TS-ED 532 is not considered to exhibit acute toxicity to zebra fish under the conditions of this test.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
0.28 mg/L

Additional information

The 96-hour acute toxicity of TS-ED 532 to zebra fish (Danio rerio) was examined in accordance with OECD Guideline for Testing of Chemicals 203, and ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 2: Semi-static method).

Dose selection was based on a preliminary study with test concentrations; 0, 10, 20, 40, 80, and 160 mg/l, where 100% mortality was observed at all concentrations. Due to the hydrophobicity of TS-ED 532 (log Kow = 6.42), the measured concentrations were much lower, including 0.28, 0.73 and 4.86 mg/L for the 1, 3, and 10 mg/L doses respectively.

Due to the low water solubility of TS-ED 532, the effect observed was considered to be a physical effect rather than a toxic effect due to partitioning of TS-ED 532 onto the gills of the fish. After 96 hours, 6 of the 10 fish dosed with the measured concentration of 0.28 mg/L were dead and all of the 10 fish in the 0.73 mg/L dose group (measured concentration) were dead. Therefore, the LC50 is >0.28 to <0.73 mg/L and the NOEC is 0.28 mg/L. The NOEC was several magnitudes higher than the estimated solubility concentration of 2 x E-4 mg/l, and TS-ED 532 was thus considered to be nontoxic within its solubility range.

The observed effect of the test substance was considered to be a physical effect rather than a toxic effect caused by partitioning of the test susbtance onto the gills of the fish.

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