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Additional toxicological data

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Administrative data

Endpoint:
additional toxicological information
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
not reported
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
In vitro toxicity of carbon nanotubes, nano-graphite and carbon black, similar impacts of acid functionalization
Author:
Figarol A, Pourchez J, Boudard D, Forest V, Akono C, Tulliani JM, Lecompte JP, Cottier M, Bernache-Assolant D, Grosseau Ph
Year:
2015
Bibliographic source:
Toxicology in Vitro 30(1 Part B), pp.476-485.

Materials and methods

Type of study / information:
Cytotoxicity
Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
The in vitro cytotoxicity of pristine and acid-treated carbon black was assessed in the RAW 264.7 murine macrophage cell line (24 hours exposure). LDH release was used for cytotoxicity assessment. The production of the cytokine TNF-α (tumour necrosis factor α) informed about the pro-inflammatory activity after a 24 h. ROS production was measured after 90 min of exposure.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Carbon black
EC Number:
215-609-9
EC Name:
Carbon black
Cas Number:
1333-86-4
Molecular formula:
C
IUPAC Name:
Carbon black
Test material form:
solid: nanoform, surface-treated

Results and discussion

Any other information on results incl. tables

Acid functionalization had no impact on LDH release and TNFalpha production. The level of ROS production was reduced after acid treatement of carbon black.

Applicant's summary and conclusion

Conclusions:
Acid treatment of carbon black induced no cytotoxic or pro-inflammatory responses in a murine macrophage cell line. These data do not support an increased cytotoxicity of carbonyl-functionalised carbon blacks.
Executive summary:

Acid functionalization had no impact on LDH release and TNFalpha production in a murine macrophage cell line. The level of ROS production was reduced after acid treatement of carbon black.