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EC number: 204-673-3 | CAS number: 124-04-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: UBA-Verfahrensvorschlag: "Letale Wirkung beim Zebrabaerbling Brachydanio rerio, LC0, LC50, LC100; 48-96 h", Guideline proposal of the German Federal Environmental Agency (UBA)
- Version / remarks:
- May 1984
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Remarks:
- ion chromatography
- Details on sampling:
- daily (0 h, 24 h, 48 h, 72 h, 96 h)
- Vehicle:
- no
- Details on test solutions:
- direct weighing, test medium (synthetic origin, prepared according to ISO)
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- - species: Zebra fish (Danio rerio)
- source: West Aquarium GmbH (Germany)
- date of birth: 15.12.1990
- date supplied: 07.03.1991
- Length: 2.5 to 3.5 cm
- Temperature: 20 - 24°C
- Dissolved oxygen: > 5 mg/L
- Mortalities during acclimatisation period: < 2 %
- Medication: None
- Keeping water: freshwater prepared after ISO - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 13° dH
- Test temperature:
- 21.8 - 22.5 °C
- pH:
- 7.4 - 7.7
- Dissolved oxygen:
- 7.6 - 8.7 mg/L
- Nominal and measured concentrations:
- Nominal concentration: 1000 mg/L
Measured concentrations: 0h = 1010; 24h = 980; 48h = 970 and 96h = 980 mg/L - Details on test conditions:
- - Test vessels: glass aquaria holding 5 L of test media covered with glass plates
- Experimental design: 1 test concentrations plus 1 control
- 10 animals per test concentration
- no feeding during the exposure period
- static system
- Method of initiation: animals were placed in prepared media
- Photoperiod: 16 h light: 8 h dark
- Temperature: 21.8 to 22.5 °C
- Test item concentration/s: 1000 mg/L (nominal concentration)
- Method of administration: direct weighing
- Duration of exposure: 96 hours
- Criteria of effects: The criterion of death used in this study was the absence of response to physical stimulation. In addition to observations on mortality at 0, 24, 48, 72 and 96 hours, type and incidence of sub-lethal effects compared with control fish were observed. - Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- 97 % of the test substance was recovered by analytical monitoring.
- Validity criteria fulfilled:
- yes
- Remarks:
- mortality of control >= 10% (or one fish, if fewer than 10 control fish are tested) at the end of exposure; dissolved oxygen concentration ≥60% of the air saturation value in all test vessels throughout the exposure; analytical measurement exists
- Conclusions:
- No toxicity of the substance toward fish was determined after 96 h under exposure conditions.
- Executive summary:
The study was conducted according UBA-Verfahrensvorschlag: "Letale Wirkung beim Zebrabaerbling Brachydanio rerio, LC0, LC50, LC100; 48-96 h" (Mai 1984) (Bayer AG, 1991) to assess the acute toxic effects of a tst substance to a freshwater fish species.
A group of ten fish was exposed to a test concentration of 1000 mg/L (nominal concentration) of test substance. Observations were made on the numbers of dead fish and the incidence of sub-lethal effects after 0, 24, 48, 72 and 96 hours of exposure.
During the test a temperature range of 20 - 24°C is to be maintained in the test vessels, with a maximum temperature fluctuation of +/- 1°C in each individual test. pH and oxygen values are measured at the beginning of the test and every 24 hours thereafter. The test is conducted as static test in which no flow of test solution occurs and solutions remain unchanged throughout the duration of the test.
It is assumed that the test solution was buffered because the pH remained between 7.4 and 7.7.No toxicity of the substance toward fish was determined after 96 h under exposure conditions.
Reference
Description of key information
The study was conducted according UBA-Verfahrensvorschlag: "Letale Wirkung beim Zebrabaerbling Brachydanio rerio, LC0, LC50, LC100; 48-96 h" (Mai 1984) (Bayer AG, 1991) to assess the acute toxic effects of the test substance to a freshwater fish species in buffered medium.
A group of ten fish was exposed to a test concentration of 1000 mg/L (nominal concentration) of test substance. Observations were made on the numbers of dead fish and the incidence of sub-lethal effects after 0, 24, 48, 72 and 96 hours of exposure.
During the test a temperature range of 20 - 24°C is to be maintained in the test vessels, with a maximum temperature fluctuation of +/- 1°C in each individual test. pH and oxygen values are measured at the beginning of the test and every 24 hours thereafter. The test is conducted as static test in which no flow of test solution occurs and solutions remain unchanged throughout the duration of the test.
It is assumed that the test solution was buffered because the pH remained between 7.4 and 7.7
No toxicity of the substance toward fish was determined after 96 h under exposure conditions.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 1 000 mg/L
Additional information
Should read "LC50 >1000mg/L"
There are studies on toxicity to fish of lower values available. The studies were discussed in the ICCA OECD/HPV-program as follows.
In a study with adipic acid, conducted according to US-EPA Method 660/3-75-009 a 96 h-LC50 of 97 mg/L for Pimephales promelas was determined. (Mattson, 1976). The authors note that the pH was < 5.9 during the test. In addition, there is no exact information on oxygen content of the test solutions. It is only reported that the oxygen content was not < 4 mg/L. Therefore, it cannot be excluded that the toxicity observed was due to pH effects and possibly oxygen limitations. A reliability score of 3 (invalid) was assigned and the study should not be used for the hazard assessment of adipic acid.
In another acute test performed with Leuciscus idus according to the German national standard method DIN 38412 Part 15 a 96 h-LC50 of 230 mg/L for adipic acid was obtained (BASF, 1980). Also in this study the pH of the test solutions was in the range of 3.8 to 7. For the concentration 215 mg/L that is in the same order of magnitude with the LC50 the pH was between 4.3 and 4.7 and therefore pH related effects cannot be excluded. Additionally, a test at a concentration of 464 mg/L in neutralised test solution was carried out. The exposure duration was not reported. No effects were observed. For this reason also this study should not be used for the hazard assessment.
Further, lower values on toxicity of adipic acid to fish are found for the species Oryzias latipes (MITI, 1997). First, a 96h-LC50 > 100 mg/L conducted according to OECD TG 204 “Fish, Acute Toxicity Test” is reported. Second, a 14d-LC50 of 80 mg/L is obtained following the OECD TG 204 “Fish, Prolonged Toxicity Test: 14-day-Study”. As no information is given on pH, the studies are stated to be reliable, but should not used for the hazard assessment, based on the given reasons resulting from the discussion above.
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