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Long-term toxicity to aquatic invertebrates

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Reference
Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1997
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Qualifier:
according to guideline
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
GLP compliance:
not specified
Analytical monitoring:
yes
Remarks:
HPLC
Details on sampling:
On day 0, 7, 14 and 18
Test organisms (species):
Daphnia magna
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Test temperature:
20 +- 1 °C
Nominal and measured concentrations:
nominal: 50.0, 25.0, 12.5, 6.25 and 3.13 mg/L (common ratio: 2.0) and control group
Details on test conditions:
- Number of organisms: 20 animals / concentration (5 animals per test vessel)
- Exposure period: 21 d
- Exposure method: Flow-through type (Continuous supply of test solution)
- Test concentration: 50.0, 25.0, 12.5, 6.25 and 3.13 mg/L (common ratio: 2.0) and control group
- Number of vessels: 4 vessels per concentration
- Test volume: Approx. 1.8 L / vessel
- Test water temperature: 20 +-1°C
- Lighting: Room light, 16 hours light (less than 1200 lux) / 8 hours dark
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
6.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
17.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Details on results:
The results relate to nominal concentrations, based on test substance concentrations.
Conclusions:
A 21d-NOEC of 6.25 mg/L was determined for the test substance towards daphnids under exposure conditions.
Executive summary:

A study was performed to assess the long-term toxicity of adipic acid to Daphnia magna STRAUS under flow-throug conditions in accordance with the OECD Guideline for Testing of Chemicals No. 211 'Daphnia magna, Reproduction Test'.

Daphnia magna were exposed to a range of concentrations (nominal: 50.0, 25.0, 12.5, 6.25 and 3.13 mg/L) and a control for 21 d. 20 daphnis (4 vessels, 5 animals/vessel) were exposed per concentration. The test temperature was 20 +- 1°C.

After 21 d a NOEC of 6.25 mg/L was determined for adipic acid towards daphnids under exposure conditions.

Description of key information

A study was performed to assess the long-term toxicity of adipic acid to Daphnia magna STRAUS under flow-throug conditions in accordance with the OECD Guideline for Testing of Chemicals No. 211 'Daphnia magna, Reproduction Test'.

Daphnia magna were exposed to a range of concentrations (nominal: 50.0, 25.0, 12.5, 6.25 and 3.13 mg/L) and a control for 21 d. 20 daphnis (4 vessels, 5 animals/vessel) were exposed per concentration. The test temperature was 20 +- 1°C.

After 21 d a NOEC of 6.25 mg/L was determined for adipic acid towards daphnids under exposure conditions.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
6.25 mg/L

Additional information

Any information on pH during the test was not reported. Following the discussion about concentration-dependant decrease of pH, it can be concluded that effects found in this study are likely not due pH effects, based on the low range of concentrations used in this test.

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