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EC number: 201-074-9 | CAS number: 77-99-6
- Life Cycle description
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- Endpoint summary
- Stability
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- Ecotoxicological Summary
- Aquatic toxicity
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Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study, GLP
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
- Deviations:
- not specified
- Principles of method if other than guideline:
- The method was reported to be on the basis of EG-Guideline 79/831 EWG annex V; OECD Guideline 301 E: Modified OECD-Screening
- GLP compliance:
- yes
- Oxygen conditions:
- not specified
- Inoculum or test system:
- activated sludge, industrial, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): STP for Laboratories, Wupperverband
- Concentration of inoculum: 0.5 mL/900mL. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 19 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Test temperature: 22+/-2°C
- Continuous darkness: yes
SAMPLING
- Sampling frequency: DOC-measurements were made on day 0, 7, 14, 21, 27 and 28.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
CALCULATION
Percentage DOC-removal = [DOC (start of the test) - DOC (end of the test)/DOC (start of the test)] x 100 - Reference substance:
- aniline
- Parameter:
- % degradation (DOC removal)
- Value:
- 6
- Sampling time:
- 28 d
- Details on results:
- Details were not provided
- Results with reference substance:
- DOC removal of reference substance (in %): 100 after 28 days.
- Validity criteria fulfilled:
- yes
- Remarks:
- Reference substance degraded by 100% within 28 days
- Interpretation of results:
- other: not readily biodegradable
- Executive summary:
A 28-d Modified Screening Test was performed on the basis of the OECD guideline 301E with predominantly domestic sewage.
6% degradation was observed within 14 and 28 days.
Trimethylolpropane is not readily biodegradable.
Reference: Müller & Caspers, 1990
Reference
Time [h] |
DOC Inoculum blank |
DOC test substance [mg/L] |
DOCcorr.* test substance [mg/L] |
DOC removal
[%] |
o |
2 |
19 |
17 |
0 |
7 |
2 |
19 |
17 |
0 |
14 |
2 |
18 |
16 |
6 |
21 |
2 |
20 |
18 |
0 |
27 |
2 |
19 |
17 |
0 |
28 |
2 |
18 |
16 |
6 |
DOCcorr.*= value for test substance - value for inoculum
Description of key information
Trimethylolpropane was shown not to be readily biodegradable, but fulfilled the criterion for inherent, ultimative biodegradability (70% degradability within 7 days).
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable, fulfilling specific criteria
- Type of water:
- freshwater
Additional information
READY BIODEGRADABILITY
Key study:
A 28-d Modified Screening Test was performed on the basis of the OECD guideline 301E with predominantly domestic sewage. 6% degradation was observed within 14 and 28 days. Trimethylolpropane is not readily biodegradable.
Reference: Müller & Caspers, 1990
Supporting studies:
A 14-d Modified MITI Test (I) was performed on the basis of the OECD guideline 301C with activated sludge. 4% degradation was observed within 14 days on the basis of O2 consumption, 3 % when estimated by test material analysis by HPLC. Trimethylolpropane is not readily biodegradable.
Reference: MITI; <= 1994
The ready biodegradability of trimethylopropane was tested in a 14 -d MITI (I)-Test following a Japanese guideline, which corresponds to OECD 301C. The test substance was investigated at a concentration of 100 mg/L. 30 mg/L of activated sludge was used as inoculum. Degradation of trimethylopropane was recorded over a period of 14 days by analyzing BOD, TOC and the active substance. Trimethylopropane was degraded to 1.7 % (based on BOD) and 3 % (from test material analysis by GC) and is therefore considered not readily biodegradable.
Reference: CITI Japan, 1992
Further studies:
A 20-d Closed Bottle Test was performed with trimethylolpropan at a concentration of 2.4 mg/L.
0% degradation was observed within 5, 10 and 20 days.Trimethylolpropane is not readily biodegradable. As only a short abstract is available, the reliability is not assignable.
Reference: Müller & Caspers, 1973
A 20-d Closed Bottle Test was performed with trimethylolpropan at concentrations of 2.4, 8, 24 and 80 mg/L.
0% degradation was observed within 20 days.Trimethylolpropane is not readily biodegradable. As the used sludge population was adapted and the available information is too scarce, the study is not reliable.
Reference: Anonymous (Bayer), 1973
INHERENT BIODEGRADABILITY
Key study:
The inherent biodegradability of trimethylolpropane was tested in a 28-d- Zahn-Wellens test following the OECD Guideline 302B (draft from 1989) and GLP requirements. With an initial test substance concentration of 100 mg/L and an activated sludge concentration of 0.4 g dry weight/L 100% degradability of trimethylolpropane was determined by DOC-removal. 100% of the reference substance aniline degraded within the test period.
Trimethylolpropane was shown to be 100% inherently biodegradable after 28 days and fulfilled the criterion for inherent, ultimative biodegradability (70% degradability within 7 days).
Reference: Caspers & Müller, 1992
Supporting studies:
The inherent biodegradability was tested in a 17 d Zahn-Wellens-Test following the OECD Guideline 302B. A 0.1% aquatic solution of the test substance was exposed to activated sludge. 95% degradation was measured on day 15 and 98% on day 17 by DOC removal. The substance is inherently biodegradable.
As only a citation of a guideline test is available, the reliabiliy cannot be assigned.
Reference: BASF, Institute Kuhlmann, 1988
FURTHER STUDIES (BOD5, BOD10 and BOD15)
The BOD5 was estimated in the study to be > 5 mg O2/mg trimethylolpropane. For the dossier compilation, a ThOD of 1.908 mg/L was calculated according the formula given in OECD-Guideline 301, Annex IV. From these results, a degradation rate from BOD x 100/ThOD may not be calculated.
As only a short abstract is available, the reliability may not be assigned.
Reference: BASF, Institute Kuhlmann, 1988
The BOD5, BOD10 and BOD15 was estimated in the study to be 0.033, 0.269 and 0.428 mg O2/mg trimethylolpropane.
For the dossier compilation, a ThOD of 1.908 mg/L was calculated according the formula given in OECD-Guideline 301, Annex IV.
From these results, the following degradation rates are calculated: ca. 1.7 % on day 5, ca. 14.1 % on day 10 and ca. 22.4 % on day 15.
As only a short abstract is available, the reliability cannot be assigned.
Reference: Walton & Davis, 1980
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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