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Ecotoxicological information

Toxicity to microorganisms

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Reference
Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Not reported
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
Deviations:
yes
Remarks:
Highest concentration tested was 10,00 g/L upon request of Study Sponsor; due to nature of the material, test temperature was 25 +/- 1°C instead of 21 +/- 1°C, cultures plated on full agar plates instead of Erlenmeyers and duration 72 h instead of 16 h
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
Maximal soluble amount of test material (saturation concentration) diluted in doubly distilled water. Test concentrations prepared by serial dilution of initial saturated solution.
Test organisms (species):
Pseudomonas putida
Details on inoculum:
Pseudomonas putida MIGULA, strain Berlin 33/2, from the German Microorganism culture Collection (D-3300 Braunschweig)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
16 h
Post exposure observation period:
None
Test temperature:
25 +/- 1°C
Nominal and measured concentrations:
- Nominal concentrations: 0.01, 0.05, 0.10, 0.50, 1.00, 2.50, 5.00 and 10.00 g/L
Details on test conditions:
- Temperature: 25 +/- 1°C
Reference substance (positive control):
no
Key result
Duration:
16 h
Dose descriptor:
EC10
Effect conc.:
ca. 830 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Key result
Duration:
16 h
Dose descriptor:
EC50
Effect conc.:
ca. 6 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition

None.

Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the study, the nominal 16 h EC50 and EC10 were 6.0 and 0.83 g/L (i.e. 6000 and 830 mg/L), respectively.
Executive summary:

A study was conducted to assess the toxicity of the test substance, C8-18 and C18-unsatd. DEA, to the bacteria Pseudomonas putida according to DIN Guideline 38412 L8, in compliance with GLP. Pseudomonas putida colonies, in triplicate, were exposed to the test substance (directly diluted in test medium) at nominal concentrations of 0.01, 0.05, 0.10, 0.50, 1.00, 2.50, 5.00 and 10.00 g/L. Inhibition of cell growth compared to controls was recorded after 16 h. Under the conditions of the study, the nominal 16 h EC50 and EC10 were 6.0 and 0.83 g/L (i.e. 6000 and 830 mg/L), respectively (Poth, 1992).

Description of key information

Key value for chemical safety assessment

EC50 for microorganisms:
6 000 mg/L
EC10 or NOEC for microorganisms:
830 mg/L

Additional information

A study was conducted to assess the toxicity of the test substance, C8-18 and C18-unsatd. DEA, to the bacteria Pseudomonas putida according to DIN Guideline 38412 L8, in compliance with GLP. Pseudomonas putida colonies, in triplicate, were exposed to the test substance (directly diluted in test medium) at nominal concentrations of 0.01, 0.05, 0.10, 0.50, 1.00, 2.50, 5.00 and 10.00 g/L. Inhibition of cell growth compared to controls was recorded after 16 h. Under the conditions of the study, the nominal 16 h EC50 and EC10 were 6.0 and 0.83 g/L (i.e. 6000 and 830 mg/L), respectively (Poth, 1992).