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Short-term toxicity to aquatic invertebrates

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Description of key information

The 48 h EC50 of  amides, C8-18 and C18-unsatd., N,N-bis(hydroxyethyl) in freshwater invertebrates is 3.2 mg/L.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
3.2 mg/L

Additional information

An acute toxicity study according to OECD guideline 202 was performed to evaluate the immobilisation potential of amides, C8-18 and C18-unsatd., N,N-bis(hydroxyethyl) inDaphnia magna.The daphnids were exposed to test substance at 0, 1, 2, 4, 8 and 16 mg/L under static conditions. Observations were performed at 0, 24 and 48 h to determine the % immobilised daphnids in the control and test dishes. The 48 h EC50value was determined to be 3.2 mg/L (Olsson T, 1994).


A study was conducted to evaluate the acute toxicity of structurally similar amides, C12-18 (even numbered) and C18-unsatd., N,N-bis(hydroxyethyl) to Daphnia magna under static conditions. The procedures followed OECD guideline 202 with deviations. Daphnia (20 per dose) were exposed to the test substance in a concentration range of 1-11 mg/L for 24 h and immobility was determined at 24 hours. Based on the results, the 24 h EC0, EC50 and EC100 were 2.0, 3.3 and 5.6 mg/L, respectively (Gode, 2001).