Registration Dossier

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
2.4 mg/L

Additional information

A study was conducted to evaluate the acute toxicity of the test substance, C8-18 and C18-unsatd. DEA, to Rainbow trout (Oncorhynchus mykiss) according to OECD Guideline 203, in compliance with GLP. Based on the results of a range-finding study, groups of 20 trout were exposed to the test substance (directly dispersed in water) at nominal concentrations of 1.0, 1.8, 3.2, 5.6 and 10 mg/L for 96 h under semi-static conditions. Mortality and adverse reactions were determined at 3, 6, 24, 48, 72 and 96 h. No analytical dose verification was conducted. Mortality occurred as of 72 h at 3.2 mg/L and as of 24 h at the two higher doses. Behavioural responses (increased pigmentation, fish swimming at the bottom of the test vessels, loss of equilibrium and presence of moribund fish) were observed as of 1.8 mg/L. Under the study conditions, the nominal 96 h LC50 and NOEC were determined to be 2.4 and 1.0 mg/L, respectively (Wetton, 1996).

A study was conducted to determine the acute toxicity of the test substance, C8-18 and C18-unsatd. DEA, to Zebra fish (Danio rerio) according to OECD Guideline 203, in compliance with GLP. Groups of 10 fish were exposed to the test substance (directly dissolved in water) at nominal concentrations of 0, 1, 2, 4, 6 and 8 mg/L for 96 h under semi-static conditions. Mortality was observed at 0, 3, 24, 48, 72 and 96 h. No analytical dose verification was conducted. Under the conditions of the study, the nominal 96 h LC50 and NOEC were calculated to be 4.9 and 2.0 mg/L, respectively (Olsson, 1994).