Dimethyl sulfoxide

Repeated dose toxicity: other routes

Administrative data

Endpoint:

sub-chronic toxicity: other route

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Descriptions of these studies were limited in detail and only the eyes were examined

Data source

Reference

Reference Type:

publication

Title:

Ocular effects of oral and dermal application of dimethyl sulfoxide in animals

Author:

Rubin LF and Barnett KC

Year:

1967

Bibliographic source:

Ann. N.-Y. Acad. Sci., 141, 333-345

Materials and methods

Principles of method if other than guideline:

Method: other

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Dimethyl sulfoxide

EC Number:

200-664-3

EC Name:

Dimethyl sulfoxide

Cas Number:

67-68-5

Molecular formula:

C2H6OS

IUPAC Name:

dimethyl sulfoxide

Details on test material:

Test compound: Dimethylsulfoxyde (spectrograde DMSO)
CAS no.: 67-68-5
Source: no data
Batch : no data
Purity : no data

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Administration / exposure

Route of administration:

other: dermal to normal and abraded skin

Vehicle:

water

Details on exposure:

50% and 90% solution of DMSO in water were applied 5 days weekly to the skin of 2 series of 5 groups of 6 male and 6 female rabbits (3 months old) at the dose levels of 0, 3 and 9 ml/kg/d. In one series solutions were applied on the intact skin and the other on the abraded skin.

Analytical verification of doses or concentrations:

not specified

Duration of treatment / exposure:

22 weeks

Frequency of treatment:

5 days/week

Doses / concentrations

Remarks:

Doses / Concentrations:
1.65 and 3 g/kg/d (as 50% or 90% DMSO solutions)

No. of animals per sex per dose:

4 rabbits per group

Control animals:

yes, concurrent vehicle

Details on study design:

Post-exposure period: none

Examinations

Observations and examinations performed and frequency:

The rabbits were examined opthalmoscopically in the 22nd dose-week, slit lamp biomicroscopy was done as well.

Results and discussion

Results of examinations

Clinical signs:

not examined

Mortality:

not examined

Body weight and weight changes:

not examined

Food consumption and compound intake (if feeding study):

not examined

Food efficiency:

not examined

Water consumption and compound intake (if drinking water study):

not examined

Ophthalmological findings:

effects observed, treatment-related

Haematological findings:

not examined

Clinical biochemistry findings:

not examined

Urinalysis findings:

not examined

Behaviour (functional findings):

not examined

Organ weight findings including organ / body weight ratios:

not examined

Gross pathological findings:

not examined

Histopathological findings: non-neoplastic:

not examined

Histopathological findings: neoplastic:

not examined

Details on results:

Lenticular changes were observed in rabbits receiving daily dermal applications of 9 ml/kg, 50% and 90% DMSO, after 15-20 weeks. After 22 weeks these changes were observed in all rabbits receiving 9 ml/kg 90% DMSO and some receiving the smallest dose administered, 3 ml/kg DMSO.
There were no data reported on local skin effects.

Effect levels

Dose descriptor:

LOAEL

Effect level:

1 650 mg/kg bw/day

Sex:

male/female

Target system / organ toxicity

Critical effects observed:

not specified