Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 200-664-3 | CAS number: 67-68-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Repeated dose toxicity: other routes
Administrative data
- Endpoint:
- short-term repeated dose toxicity: other route
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- publication
- Title:
- Subacute toxicity of intravenous dimethyl sulfoxide in rhesus monkeys
- Author:
- de la Torre JC, Surgeon JW, Ernest T, Wollmann R
- Year:
- 1 981
- Bibliographic source:
- J. Toxicol. Environ. Health, 7 (1), 49-57
Materials and methods
- Principles of method if other than guideline:
- Method: other: no data
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Dimethyl sulfoxide
- EC Number:
- 200-664-3
- EC Name:
- Dimethyl sulfoxide
- Cas Number:
- 67-68-5
- Molecular formula:
- C2H6OS
- IUPAC Name:
- dimethyl sulfoxide
- Details on test material:
- Test compound: Dimethylsulfoxyde
CAS no.: 67-68-5
Source: Research Indistries, Salt Lake City, Utah
Batch : no data
Purity : 100% pyrogen free
Constituent 1
Test animals
- Species:
- monkey
- Strain:
- other: Macaca mulatta (rhesus)
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- Wieght: 3-6 kg
Administration / exposure
- Route of administration:
- intravenous
- Vehicle:
- physiological saline
- Duration of treatment / exposure:
- 9 days consecutive
- Frequency of treatment:
- 1 x per day
Doses / concentrations
- Remarks:
- Doses / Concentrations:
3 and 2 g/kg
- No. of animals per sex per dose:
- 6 males
- Control animals:
- yes, concurrent vehicle
- Details on study design:
- Post-exposure period: 120 days
Daily i.v. doses of 3 or 2 g/kg DMSO in a 40% solution were given respectively to 4 or 1 male rhesus monkeys for 9 consecutive days.
Examinations
- Observations and examinations performed and frequency:
- The monkeys were monitored before and after treatment for 4 months for changes in blood chemistry, haematology, urine, and ocular, neurological, and cardiovascular systems.
At the end of the study all animals were sacrificed and gross and microscopic pathological examinations were performed.
Results and discussion
Results of examinations
- Clinical signs:
- no effects observed
- Mortality:
- no mortality observed
- Body weight and weight changes:
- no effects observed
- Food consumption and compound intake (if feeding study):
- not specified
- Food efficiency:
- not specified
- Water consumption and compound intake (if drinking water study):
- not specified
- Ophthalmological findings:
- no effects observed
- Haematological findings:
- no effects observed
- Clinical biochemistry findings:
- no effects observed
- Urinalysis findings:
- no effects observed
- Behaviour (functional findings):
- not examined
- Organ weight findings including organ / body weight ratios:
- no effects observed
- Gross pathological findings:
- no effects observed
- Histopathological findings: non-neoplastic:
- no effects observed
- Histopathological findings: neoplastic:
- not examined
- Details on results:
- The blood chemistries, urinalysis, haematology, and ophthalmologic and gross and histological examinations of tissues in the DMSO animals were not significantly different from those in the control monkey.
The rapid four-fold increase in diuresis over the control values in the monkeys receiving 2 or 3 g/kg DMSO supports previous results. This urine output indicates that DMSO is one of the strongest diuretics known. The absence of any microscopic or gross structural damage to the kidneys suggests that the coffee-colored urine seen after DMSO administration represents a transient erythrocytic hemolysis, possibly caused by an osmotic gradient due to DMSO in the vascular system. Since this effect was short lived and did not significantly alter the red cell count or the haemoglobin and hematocrit values, the coffee-colored urine represented a transient and reversible condition.
The partial thromboplastin time in DMSO treated monkeys decreased from a normal mean of 46 s to a mean of 18.7 s. These values returned to normal when DMSO administration was stopped. Although the PT values in both saline and DMSO treated monkeys fluctuated slightly before and after treatment, the values did not significantly differ from the control standard range. By contrast, the PTT values in both DMSO and saline treated animals were abnormal even before treatments began and remained that way after treatment.
An increase in respiratory rate after DMSO was previously reported. It appears from these studies that DMSO acts as a central respiratory stimulant when given iv in bolus form. A rise in minute respiratory volume concomitant with the rise in respiratory rate was also reported.
Target system / organ toxicity
- Critical effects observed:
- not specified
Any other information on results incl. tables
These data indicate that monkeys receiving a total of 108-127g 40% DMSO or equal saline volumes iv during a 9-d study showed no significant or adverse changes in the chemical and physiological parameters studied. No gross or microscopic pathology was found in any monkey at the end of the 4-mo observation period.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

EU Privacy Disclaimer
This website uses cookies to ensure you get the best experience on our websites.