Registration Dossier
Registration Dossier
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EC number: 200-664-3 | CAS number: 67-68-5
Dermal absorption
Administrative data
- Endpoint:
- dermal absorption in vivo
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Adsorption, distribution and metabolism of dimethylsulfoxide in the rat, rabbit and guinea pig
- Author:
- Hucker HB, Ahmad PM and Miller EA
- Year:
- 1 966
- Bibliographic source:
- J. Pharm. Exp. Ther, 154, 176-184
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Absorption of DMSO-S35
- GLP compliance:
- no
Test material
- Reference substance name:
- Dimethyl sulfoxide
- EC Number:
- 200-664-3
- EC Name:
- Dimethyl sulfoxide
- Cas Number:
- 67-68-5
- Molecular formula:
- C2H6OS
- IUPAC Name:
- dimethyl sulfoxide
- Details on test material:
- Test compound Dimethylsulfoxyde
Source: New England Nuclear Corp.
Specific activity: 71 µCi/mmol
CAS no.: 67-68-5
Cold DMSO
Source: Crown-Zellerbach
Batch : no data
Purity : pure by CG
DMSO-S35
Source: New England Nuclear Corp.
Purity: Pure by IR spectroscopy and GC
Specific activity: 71 µc/mmole
Constituent 1
- Radiolabelling:
- yes
- Remarks:
- DMSO-S[35]
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male
Administration / exposure
- Vehicle:
- water
- Doses:
- 550mg/kg (0.5-11µC)
- No. of animals per group:
- 8 males
- Details on study design:
- Absorption, distribution and metabolism of DMSO have been studied in rats using S35-labeled DMSO.
Male Sprague Dawley rats received 0.55 g/kg (0.5-11 µCi) of S35-DMSO dermally. Animals were enclosed in wire screen by folding it over the animal and stapling it on the three sides to prevent oral ingestion. DMSO was the napplied by means of a syringe and needle through an opening cut in the screen over a shaved area on the back. Possible stress effects were minimized by restricting confinment time usually to 1h and never longer than 4 h.
Two animals each were sacrificed after 30 min, 1 hr, 2 hr and 24 hr respectively. The animals were ether-treated and tissues were removed, blotted, weighed and homogenized.
Results and discussion
- Absorption in different matrices:
- After 30 min, 63% (average) of the dose remained at the site of application; after 1 hr, 19% (average) of the dose remained; and, after 2 hr, 14% (average) was left.
After 24 hr, the radioactivity at the site of application was the same as that of the surrounding skin. .
Percutaneous absorption
- Remarks on result:
- other:
Applicant's summary and conclusion
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