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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
April - May 1980
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Basic data given
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Unnamed
Year:
1989
Reference Type:
secondary source
Title:
Chloroethane CAS: 75-00-3
Author:
OECD SIDS
Year:
2006
Bibliographic source:
SIDS Initial Assessment Report for SIAM 22

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
GLP compliance:
yes
Test type:
fixed concentration procedure
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Chloroethane
EC Number:
200-830-5
EC Name:
Chloroethane
Cas Number:
75-00-3
Molecular formula:
C2H5Cl
IUPAC Name:
chloroethane
Details on test material:
- Name of test material (as cited in study report): chloroethane
- Physical state: gaseous
- Analytical purity: > 99.5% (Matheson Gas Products, East Rutherford, NJ, USA)
- Lot/batch No.: 44480

Test animals

Species:
mouse
Strain:
B6C3F1
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Charles River Breeding Laboratories (Portage, MI, USA)
- Age at study initiation: 9-10 weeks
- Housing: individually in stainless steel wire cages (Harford Metal Inc., Aberdeen, MD, USA)
- Diet: NIH 07 rat and mouse ration (Zeigler Bros., Inc., Gardners, PA, USA) available ad libitum during non-exposure periods
- Water: automatic watering system (Edstrom Industries, Waterford, WI, USA), available ad libitum
- Acclimation period: 26 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): exposure: 23.9 - 24.4 °C; non-exposure: 22.2 - 24.4 °C
- Humidity (%): exposure: 55 - 57%; non-exposure: 40 - 60%
- Air changes (per hr): 10
- Photoperiod (hrs dark / hrs light): 12/12


IN-LIFE DATES: From: April 1980 To: 12 May 1980

Administration / exposure

Route of administration:
inhalation: gas
Type of inhalation exposure:
whole body
Vehicle:
air
Details on inhalation exposure:
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: vapour generation system; the liquid to be vaporized was forced under pressure, at a metered rate, directly from the shipping container into a stainless steel boiler that was maintained at about 32 °C by a controlled-temperature water bath. The vapour was routed through a gas metering valve and a purge/expose valve into a pipe at the chamber inlet, where the vapour was mixed with dilution air entering the chamber.


Analytical verification of test atmosphere concentrations:
no
Duration of exposure:
4 h
Concentrations:
19000 ppm (50130 mg/m3)
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: observed continually during exposure and then 3 times per day for 14 days; weighed initially
- Necropsy of survivors performed: no

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 19 000 ppm
Based on:
test mat.
Exp. duration:
4 h
Mortality:
All mice survived.
Clinical signs:
other: No clinical signs of toxicity were seen.

Applicant's summary and conclusion

Interpretation of results:
other: CLP/EU GHS criteria not met, no classification required according to Regulation (EC) No 1272/2008
Executive summary:

Groups of male and female mice (5/sex) were exposed by whole body inhalation for a single 4-hour exposure to air containing chloroethane at the target concentration of 19000 ppm (National Toxicology Program, 1989). Animals were weighed before exposure and were observed continually during exposure and then three times per day for 14 days. After 14 days, the animals were killed without a formal necropsy. All mice survived the 4-hour exposure to 19000 ppm chloroethane. No clinical signs of toxicity were seen.