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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
24 AUG - 26 AUG 1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP - Guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Ethylchlorid was given in excess in VE-water. The vessel was closed with a septum and incubated over 4 days at 4°C.
Preparation of test concentrations: head-space-vessels of 20 mL volume were filled with 19 mL synthetic freshwater and closed with as septum. All used instruments were cooled to 8°C. Adequate amounts of the stock solution were injected to each of the test vessels. The whole process was conducted at 8°C
- Controls: water control
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: waterflea
- Strain: clon 5
- Source: in-house culture
- Age at study initiation (mean and range, SD): below 24 h
- Method of breeding: parent daphnids were cultivated in Elendt medium in 1 L glass beakers. Animals were fed daily with Scenedesmus subspicatus.
- Feeding during test: no feeding

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20 +/- 1 °C
pH:
7.0 - 7.2 at test end
Dissolved oxygen:
8.3 - 8.6 at test end
Nominal and measured concentrations:
nominal: 0, 10, 20, 40, 80, 160 and 320 mg/L
mean measured: 0, 5.7, 11.1, 24.5, 46.5, 91, 217 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: round-bottom flasks filled with 10 mL test solution
- Aeration: none
- Type of flow-through (e.g. peristaltic or proportional diluter): static test
- No. of organisms per vessel: approximately 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 4 mL per test organism


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic freshwater. Composition: 294 mg/L CaCl2 x 2H2O; 123 mg/L MgSO4 x 7 H2O, 63 mg/L NaHCO3 and 5.5 mg/L KCl
- Ca/mg ratio: 4 : 1
- Intervals of water quality measurement: 48 h


OTHER TEST CONDITIONS
- Photoperiod: continuous darkness

Reference substance (positive control):
yes
Remarks:
potassium dichromate was tested in a separate study
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
58 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% C.L. 44 mg/L - 77 mg/L
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
91 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):

- Mortality: 0.9 mg/L - 25% immobile daphnids after 24 h; 1.9 mg/L - 100% immobile daphnids after 24 h

Mean measured concentration
[mg/L]

Test period (h)

24 h

48 h

Immobility [%]

Immobility [%]

0

0

0

5.7

0

0

11.1

5

5

24.5

0

7

46.5

15

20

91

50

85

217

100

100

Description of key information

EC50(48 h): 58 mg/L (meas. geom. mean)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
58 mg/L

Additional information

The acute toxicity of chloroethane (CAS 75-00-3) to aquatic invertebrates was tested in a guideline study following EU method C2. Daphnia magna were exposed to measured test concentrations of 5.7, 11.1, 24.5, 46.5, 91 and 217 mg/L under static conditions. The test concentrations were prepared by giving ethylchlorid in excess in VE-water. The vessel was closed with a septum and incubated over 4 days at 4°C. The test concentrations were prepared by filling head-space-vessels (20 mL volume) with 19 mL synthetic freshwater. The vessels were closed with a septum. At test termination an EC50 (48 h) of 58 mg/L (measured, geom. mean) were determined.

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