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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study, acceptable for assessment.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1975
Report date:
1975
Reference Type:
other company data
Title:
Unnamed
Year:
2002
Report date:
2002

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Deviations:
yes
Remarks:
Only 0.05 mL applied (instead of 0.1 mL), observation period: only 8 days (instead of 21 days)
Principles of method if other than guideline:
The study was conducted following a protocol based on BASF-internal standards.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
N,N,N',N',N'',N''-hexamethyl-1,3,5-triazine-1,3,5(2H,4H,6H)-tripropanamine
EC Number:
240-004-1
EC Name:
N,N,N',N',N'',N''-hexamethyl-1,3,5-triazine-1,3,5(2H,4H,6H)-tripropanamine
Cas Number:
15875-13-5
Molecular formula:
C18H42N6
IUPAC Name:
(3-{3,5-bis[3-(dimethylamino)propyl]-1,3,5-triazinan-1-yl}propyl)dimethylamine
Details on test material:
Name of the test substance used in the study report: Triazin (= HM 17 994)

Test animals / tissue source

Species:
rabbit
Strain:
Vienna White

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: untreated eye of the same animal
Amount / concentration applied:
0.05 ml
Duration of treatment / exposure:
once
Observation period (in vivo):
8 d
Number of animals or in vitro replicates:
2
Details on study design:
REMOVAL OF TEST SUBSTANCE
- not rinsed

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
animal #1
Time point:
other: 24-48 h
Score:
3
Max. score:
4
Reversibility:
not reversible
Remarks on result:
other: no assessment was possible after 72 h due to a clotted eye
Irritation parameter:
cornea opacity score
Basis:
animal #2
Time point:
other: 24-48 h
Score:
3
Max. score:
4
Reversibility:
not reversible
Remarks on result:
other: no assessment was possible after 72 h due to a clotted eye
Irritation parameter:
chemosis score
Basis:
animal #1
Time point:
other: 24-48 h
Score:
1
Max. score:
4
Reversibility:
not reversible
Remarks on result:
other: no assessment was possible after 72 h due to a clotted eye
Irritation parameter:
chemosis score
Basis:
animal #2
Time point:
other: 24-48 h
Score:
1
Max. score:
4
Reversibility:
not reversible
Remarks on result:
other: no assessment was possible after 72 h due to a clotted eye
Irritation parameter:
iris score
Basis:
animal #1
Time point:
other: 24-48 h
Score:
0.5
Max. score:
2
Reversibility:
not reversible
Remarks on result:
other: no assessment was possible after 72 h due to a clotted eye
Irritation parameter:
iris score
Basis:
animal #2
Time point:
other: 24-48 h
Score:
1.5
Max. score:
2
Reversibility:
not reversible
Remarks on result:
other: no assessment was possible after 72 h due to a clotted eye
Irritation parameter:
conjunctivae score
Basis:
animal #1
Time point:
other: 24-48 h
Score:
1.5
Max. score:
3
Reversibility:
not reversible
Remarks on result:
other: no assessment was possible after 72 h due to a clotted eye
Irritation parameter:
conjunctivae score
Basis:
animal #2
Time point:
other: 24-48 h
Score:
2
Max. score:
3
Reversibility:
not reversible
Remarks on result:
other: no assessment was possible after 72 h due to a clotted eye

Any other information on results incl. tables

Table 1: Individual findings after investigation of animals for eye irritation after exposure to test substance:

 

Readings

Animals

Cornea

Iris

Conjunctiva

 

 

Additional findings

 

 

Opacity

 

Redness

Chemosis

Discharge

 

24 h

1

3

-

1

1

-

nictitating membrane reddish brown, pus

2

3

1

2

1

-

scleral vessels injected, nictitating membrane grey, mucous membrane reddish brown, lubricious overlay

48 h

1

3

1

2

1

-

Ciliar injection, eye clotted, pus formation, nictitating membrane grey, mucous membrane reddish brown

2

3

2

2

1

-

Iritis, eye clotted, pus formation, nictitating membrane grey, mucous membrane reddish brown

72 h

1

 

 

 

 

 

eye clotted, no observation possible

2

 

 

 

 

 

eye clotted, no observation possible

Mean 24-48 h

1

3

0.5

1.5

1

-

 

 

2

3

1.5

2

1

-

 

Mean

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

8 d

1

3

2

2

1

-

Iritis, nictitating membrane and mucous membrane white and thick, staphyloma, pus formation

 

2

3

2

1

1

-

Iritis, nictitating membrane partly white, mucous membrane reddish brown, staphyloma, lubricious overlay

Applicant's summary and conclusion

Interpretation of results:
Category 1 (irreversible effects on the eye)
Remarks:
Migrated information
Conclusions:
The substance induced irreversible effects on the eye.
Executive summary:

The substance induced irreversible effects on the eye.