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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study, acceptable for assessment.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1975
Report date:
1975
Reference Type:
other company data
Title:
Unnamed
Year:
2002
Report date:
2002

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Deviations:
yes
Remarks:
Only 0.05 mL applied (instead of 0.1 mL), observation period: only 8 days (instead of 21 days)
Principles of method if other than guideline:
The study was conducted following a protocol based on BASF-internal standards.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
N,N,N',N',N'',N''-hexamethyl-1,3,5-triazine-1,3,5(2H,4H,6H)-tripropanamine
EC Number:
240-004-1
EC Name:
N,N,N',N',N'',N''-hexamethyl-1,3,5-triazine-1,3,5(2H,4H,6H)-tripropanamine
Cas Number:
15875-13-5
Molecular formula:
C18H42N6
IUPAC Name:
3,3',3''-(1,3,5-triazinane-1,3,5-triyl)tris(N,N-dimethylpropan-1-amine)
Details on test material:
Name of the test substance used in the study report: Triazin (= HM 17 994)

Test animals / tissue source

Species:
rabbit
Strain:
Vienna White

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: untreated eye of the same animal
Amount / concentration applied:
0.05 ml
Duration of treatment / exposure:
once
Observation period (in vivo):
8 d
Number of animals or in vitro replicates:
2
Details on study design:
REMOVAL OF TEST SUBSTANCE
- not rinsed

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
animal #1
Time point:
other: 24-48 h
Score:
3
Max. score:
4
Reversibility:
not reversible
Remarks on result:
other: no assessment was possible after 72 h due to a clotted eye
Irritation parameter:
cornea opacity score
Basis:
animal #2
Time point:
other: 24-48 h
Score:
3
Max. score:
4
Reversibility:
not reversible
Remarks on result:
other: no assessment was possible after 72 h due to a clotted eye
Irritation parameter:
chemosis score
Basis:
animal #1
Time point:
other: 24-48 h
Score:
1
Max. score:
4
Reversibility:
not reversible
Remarks on result:
other: no assessment was possible after 72 h due to a clotted eye
Irritation parameter:
chemosis score
Basis:
animal #2
Time point:
other: 24-48 h
Score:
1
Max. score:
4
Reversibility:
not reversible
Remarks on result:
other: no assessment was possible after 72 h due to a clotted eye
Irritation parameter:
iris score
Basis:
animal #1
Time point:
other: 24-48 h
Score:
0.5
Max. score:
2
Reversibility:
not reversible
Remarks on result:
other: no assessment was possible after 72 h due to a clotted eye
Irritation parameter:
iris score
Basis:
animal #2
Time point:
other: 24-48 h
Score:
1.5
Max. score:
2
Reversibility:
not reversible
Remarks on result:
other: no assessment was possible after 72 h due to a clotted eye
Irritation parameter:
conjunctivae score
Basis:
animal #1
Time point:
other: 24-48 h
Score:
1.5
Max. score:
3
Reversibility:
not reversible
Remarks on result:
other: no assessment was possible after 72 h due to a clotted eye
Irritation parameter:
conjunctivae score
Basis:
animal #2
Time point:
other: 24-48 h
Score:
2
Max. score:
3
Reversibility:
not reversible
Remarks on result:
other: no assessment was possible after 72 h due to a clotted eye

Any other information on results incl. tables

Table 1: Individual findings after investigation of animals for eye irritation after exposure to test substance:

 

Readings

Animals

Cornea

Iris

Conjunctiva

 

 

Additional findings

 

 

Opacity

 

Redness

Chemosis

Discharge

 

24 h

1

3

-

1

1

-

nictitating membrane reddish brown, pus

2

3

1

2

1

-

scleral vessels injected, nictitating membrane grey, mucous membrane reddish brown, lubricious overlay

48 h

1

3

1

2

1

-

Ciliar injection, eye clotted, pus formation, nictitating membrane grey, mucous membrane reddish brown

2

3

2

2

1

-

Iritis, eye clotted, pus formation, nictitating membrane grey, mucous membrane reddish brown

72 h

1

 

 

 

 

 

eye clotted, no observation possible

2

 

 

 

 

 

eye clotted, no observation possible

Mean 24-48 h

1

3

0.5

1.5

1

-

 

 

2

3

1.5

2

1

-

 

Mean

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

8 d

1

3

2

2

1

-

Iritis, nictitating membrane and mucous membrane white and thick, staphyloma, pus formation

 

2

3

2

1

1

-

Iritis, nictitating membrane partly white, mucous membrane reddish brown, staphyloma, lubricious overlay

Applicant's summary and conclusion

Interpretation of results:
Category 1 (irreversible effects on the eye)
Remarks:
Migrated information
Conclusions:
The substance induced irreversible effects on the eye.
Executive summary:

The substance induced irreversible effects on the eye.