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Short-term toxicity to fish

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In a static short-term toxicity to fish test according to DIN 38 412 – L15 guideline (equivalent to OECD 203), the 96-hour LC50 of the test substance for Leusciscus idus (Golden Orfe) was determined to be nominal in the range of 100 to 220 mg/L. 

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In a static short-term toxicity to fish test according to DIN 38 412 – L15 (1982) guideline (equivalent to OECD 203), Leusciscus idus (Golden Orfe) were exposed to the test item for 48 hours. The test substance was tested in the nominal concentrations of 46.4, 100, 215, 464 mg/L. The 48h-LC50 was determined to be nominal in the range of 100 – 220 mg/L. No analytical monitoring was done. In addition to this, to study the effect of the high pH on the toxicity the concentration 464 mg/L was tested in parallel after pH-adjustment using hydrochlorid acid. After pH adjusted 10 % (1/10) fish were dead over 96 hours of a concentration of 464 mg/L.

In addition to that a supporting study with the surrogate test substance Diethylaminopropylamin according to DIN 38 412 – L15 (1982) guideline (equivalent to OECD 203) was performed on Leusciscus idus over 48 hours. The 48h-LC50 was also determined to be nominal in the range of 100 – 220 mg/L. No analytical monitoring was done. In addition to this, to study the effect of the high pH on the toxicity the concentration 464 mg/L was tested in parallel after pH-adjustment using hydrochlorid acid. After pH adjusted no fish were dead over 96 hours of a concentration of 464 mg/L.