Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

The ready biodegradability of the surrogate test substance 3-Diethylaminopropylamine was determined according to the OECD guideline 301A (1993). The test substance was biodegraded by an average of 90-100 % over the test period of 28 days. Under the test conditions the surrogate test substance was found to be ready biodegradable according to OECD criteria. 

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

The ready biodegradability of the surrogate test substance 3-Diethylaminopropylamine was determined according to the OECD guideline 301A (1993). The biodegradation of the surrogate test substance was followed by exposing it to the municipal activated sludge from the waste water treatment plant of Mannheim/Baden-Württemberg (Germany), stored in Laboratory wastewater treatment plant which was fed with municipal sewage. The sludge was washed and aerated for 24 hours and then added to the test vessels to obtain a sludge concentration of 30 mg/L dry substance. The control substance (aniline) was biodegraded by an average of 90-100 % over 14 days. Under the test conditions the surrogate test substance 3 -diethylaminopropylamine was found to be ready biodegradable over the test period of 28 days. Furthermore, the test substance showed no inhibitory effect on the microorganisms. At the end of the test, the surrogate test substance 3 -diethylaminopropylamine was biodegraded by an average of 90-100 %, therefore the validity criteria was fulfilled.

In addition a Non GLP study according to the OECD guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test) with the test substance has also been performed. The biodegradation of the test substance was followed by exposing it to the activated sludge from industrial waste water treatment plant over 28 days. According to this OECD 302B test, the test item is easily removed from water. But since the activated sludge used in this test is from industrial waste water treatment plant instead of municipal wastewater treatment plant, it is not possible to make a conclusion concerning the biodegradability of the substance. The test item was degraded 84 % after 28 days.

Another test with the test read across substance 3 -aminopropyldiethylamine according to OECD 302 B was also performed. This read across substance was degraded 96 % after 18 days.