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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Description of key information

In a key static growth inhibition test, the fresh algae Pseudokirchnerella subcapitata was exposed over 72 hours to the surrogate test substance 3-aminopropyldiethylamine according to the OECD guideline 201 and under GLP conditions. The 72h-ErC50 and the 72h-NOEC were determined to be 34 and 19.53 mg/L, respectively. The 72h-ErC10 was determined to be 26 mg/L. The validity criteria of the study were fulfilled 
 

Key value for chemical safety assessment

EC50 for freshwater algae:
34 mg/L
EC10 or NOEC for freshwater algae:
26 mg/L

Additional information

In a key static growth inhibition test, the fresh algae Pseudokirchnerella subcapitata was exposed over 72 hours to the read across substance Diethylaminopropylamine according to the OECD guideline 201 and under GLP conditions. The algae were exposed to the following nominal concentrations for 72 hours: 0, 4.77, 7.63, 12.21, 19.53, 31.25 and 50 mg/L. The final concentrations of the read across substance diethylaminopropylamine were maintained within the designated limit of 80% of the initial concentrations in non inoculated flasks. Therefore, the effect concentrations and NOEC have been calculated using the nominal concentrations. The 72h-ErC50 and the 72h-NOEC were determined to be 34 and 19.53 mg/L, respectively. The 72h-ErC10 was determined to be 26 mg/L. The validity criteria of the study were fulfilled

 

As a supportive study, acute toxicity to Desmodesmus subspicatus was investigated according to German Industrial Standard DIN 38 412/9. The 72h EC50 was determined to be between 100 and 150 mg/L. The concentrations were not measured in the vessels during the test but the read across substance Diethylaminopropylamine is expected to be stable in water as showed in the key test. Moreover, the read across substance Diethylaminopropylamine is miscible in water, its vapor pressure is low and it is not subject to hydrolysis. Therefore there are no cause of significant deviation from nominal to real substance concentrations. The samples were not neutralized.

Another test was conducted according to German Industrial Standard DIN 38 412/9. But the stock solution of the read across substance Diethylaminopropylamine was neutralised with 1N HCl to pH7. In this study, the 72h-ErC10 and the 72h-ErC50 were determined to be 3.45 g/L and 7.12 g/L, respectively. The result of the study suggests that the toxicity of the read across substance Diethylaminopropylamine is related to the increase of the medium pH but not to a specific mode of toxic action.