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Ecotoxicological information

Toxicity to microorganisms

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Description of key information

Depending on local conditions and existing concentrations, disturbances in the biodegradation process of activated sludge are possible.

Key value for chemical safety assessment

EC50 for microorganisms:
410 mg/L
EC10 or NOEC for microorganisms:
4 mg/L

Additional information

The assessment of the toxicity of 3,3'-iminodi(propylamine) (CAS 56 -18 -8) to aquatic microorganisms is based on experimental data from a respriation inhibition test according to OECD 209, performed under GLP conditions (BASF SE 2011, report no. 08G0696/05G004). Domestic activated sludge was used as inoculum. The 3h-EC50 was determined to be 410 mg/L, the 3h-EC20 was 20 mg/L and the 3h-EC10 was 4 mg/L, based on nominal concentrations.

The results of two additional studies with sludge microorganisms are available:

- In a growth inhibition study with Pseudonomas putida performed according to the German industrial test guideline DIN 38412, Part 8, an 17h-EC50 of 33 mg/L was measured (BASF, 1989, report no. 9/89/9/0499).

- In a short term respiration study with industrial activated sludge performed similar to OECD 209, an EC50 of > 750 mg/L was observed (BASF, 1979, report no. 559).

In all three tests, the concentrations were not analytically verified, but 3,3'-iminodi(propylamine) is expected to be stable in water, as the substance is miscible in water (Gestis database 2012), its vapor pressure is moderate (VP = 1.26 Pa @20°C, BASF AG 1987, report no. 187.0002.1) and its Henry's Law Constant is low (HLC = 5.54 E-08 Pa m³/mol @ 25°C, calculated with HENRYWIN v3.20, not in applicability domain, BASF SE 2017).

In conclusion, depending on local conditions and existing concentrations, disturbances in the biodegradation process of activated sludge are possible.