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EC number: 200-261-2 | CAS number: 56-18-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Endpoint summary
Administrative data
Description of key information
Additional information
Short-term toxicity to fish
With high probability, acutely not harmful to fish
A 96 -h acute toxicity study was conducted with 3,3'-iminodi(propylamine) (CAS 56 -18 -8) according to the German industrial test guideline DIN 38412, Part 15 (BASF AG 1979, report no. 79/42). The test was conducted with Leuciscus idus exposed to concentrations ranging from 100 to 464 mg/L in a static test system. The pH values of the test solutions were not adjusted. In addition, a test concentration of 500 mg/L was tested for 72 h with neutralised test solutions. The LC50 for the non-neutralised test solutions was determined to be between 215 and 316 mg/L. After 72 h, the concentration of 500 mg/L showed no mortality after neutralisation with H2SO4. Hence, observed effects were caused by the pH shift in the test medium.
The concentrations were not analytically verified during the test, but 3,3'-iminodi(propylamine) is expected to be stable in water, as the substance is miscible in water (Gestis database 2012), its vapor pressure is moderate (VP = 1.26 Pa @20°C, BASF AG 1987, report no. 187.0002.1) and its Henry's Law Constant is low (HLC = 5.54 E-08 Pa m³/mol @ 25°C, calculated with HENRYWIN v3.20, not in applicability domain, BASF SE 2017).
Long-term toxicity to fish
In Annex IX of Regulation (EC) No 1907/2006, it is laid down that chronic tests shall be proposed by the registrant if the chemical safety assessment indicates the need to investigate further the effects on fish. According to Annex I of this regulation, the chemical safety assessment triggers further action when the substance or the preparation meets the criteria for classification as dangerous according to Directive 67/548/EEC, Directive 1999/45/EC or CLP regulation (EC) no. 1272/2008, or is assessed to be a PBT or vPvB. The hazard assessment of the substance reveals neither a need to classify the substance as dangerous to the environment, nor is it a PBT or vPvB substance, nor are there any further indications that the substance may be hazardous to the environment. Considering the possibility for the prediction of relative species sensitivities according to the REACH Guidance Document R.7b, chapter R.7.8.5.3, further testing on fish would not contribute to improve the current knowledge of the substance and/or its associated risk when released to the aquatic compartment. This conclusion is based on a factor of greater than 10 for fish to aquatic invertebrates. Long-term toxicity testing is not triggered by the results of the exposure assessment and the risk characterization. The risk-characterisation ratios (RCR) do not indicate a risk for the environment (RCR < 1). Therefore, and for reasons of animal welfare, a chronic test in fish is not provided.
Short-term toxicity to aquatic invertebrates
Acutely harmful to aquatic invertebrates
To determine the short-term toxicity of 3,3'-iminodi(propylamine) (CAS 56 -18 -8) to aquatic invertebrates, a static 48 -h acute Daphnia magna toxicity study was conducted according to EU Method C2 (BASF AG 1989, report no. 1/0499/2/89). The nominal test concentrations ranged from 7.81 to 500 mg/L. The pH value of the test solutions was not adjusted. The 48 -h EC50 was estimated to be 37.4 mg/L, based on nominal concentrations.The highest test concentration of 500 mg/L was also tested after neutralising the test solution, but no significant differences to not-neutralised conditions were found.
The concentrations were not analytically verified during the test, but 3,3'-iminodi(propylamine) is expected to be stable in water, as the substance is miscible in water (Gestis database 2012), its vapor pressure is moderate (VP = 1.26 Pa @20°C, BASF AG 1987, report no. 187.0002.1) and its Henry's Law Constant is low (HLC = 5.54 E-08 Pa m³/mol @ 25°C, calculated with HENRYWIN v3.20, not in applicability domain, BASF SE 2017).
Long-term toxicity to aquatic invertebrates
Based on the GHS, no chronic toxicity is anticipated at an NOEC or EC10 > 1 mg/L.
Experimental data on the chronic toxicity of 3,3'-iminodi(propylamine) (CAS 56-18-8) on aquatic invertebrates are not available.
Read-across data on long-term toxicity are available from a GLP study according to EU method C.20 (Daphnia magna reproduction test) with the structural analogous substance DETA (CAS 111-40-0). The 21-d NOEC was determined to be 5.6 mg/L (nominal; Akzo, 1991). The concentrations were not analytically verified during the test, but DETA is expected to be stable, due to a high water solubility (miscible), a moderate vapour pressure (VP = 0.35 Pa @20°C, measured) and a low Henry's Law Constant (HLC = 3.14 E-08 Pa m³/mol @ 25°C, calculated with HENRYWIN v3.20, not in applicability domain).
The key study is supported by an OECD 211 GLP-guideline study (recorded in the ECHA database) with the structural analogous substance 1,2 -Ethanediamine, N,N'-bis(2 -aminoethyl)- (CAS 112 -24 -3), providing a 21-d EC10 of 1.9 mg/L.
Toxicity to aquatic algae
With high probability, not harmful to aquatic algae and cyanobacteria.
A study was performed according to the German industrial test guideline DIN 38412, Part 9 regarding the toxic effect of 3,3'-iminodi(propylamine) (CAS 56 -18 -8) towards the algal species Desmodesmus subspicatus (BASF AG 1989, report no. 01/0499/2/89). The endpoint were recalculated using the program ToxRatPro (v2.10; BASF SE 2011). The study is considered valid. Based on growth rate, the 72h-ErC50 was determined to be 1494.5 mg/L, and the ErC10 was determined to be 294.3 mg/L (nominal; values based on neutralised test solutions).
The concentrations were not analytically verified during the test, but 3,3'-iminodi(propylamine) is expected to be stable in water, as the substance is miscible in water (Gestis database 2012), its vapor pressure is moderate (VP = 1.26 Pa @20°C, BASF AG 1987, report no. 187.0002.1) and its Henry's Law Constant is low (HLC = 5.54 E-08 Pa m³/mol @ 25°C, calculated with HENRYWIN v3.20, not in applicability domain, BASF SE 2017).
Toxicity to microorganisms
Depending on local conditions and existing concentrations, disturbances in the biodegradation process of activated sludge are possible.
The assessment of the toxicity of 3,3'-iminodi(propylamine) (CAS 56 -18 -8) to aquatic microorganisms is based on experimental data from a respriation inhibition test according to OECD 209, performed under GLP conditions(BASF SE 2011, report no. 08G0696/05G004).Domestic activated sludge was used as inoculum. The 3h-EC50 was determined to be 410 mg/L, the 3h-EC20 was 20 mg/L and the 3h-EC10 was 4 mg/L, based on nominal concentrations.
The result is supported by two additional studies with sludge microorganisms:
In a growth inhibition study with Pseudonomas putida performed according to the German industrial test guideline DIN 38412, Part 8, an 17h-EC50 of 33 mg/L was measured (BASF, 1989, report no. 9/89/9/0499).
In a short term respiration study with industrial activated sludge performed similar to OECD 209, an EC50 of > 750 mg/L was observed (BASF, 1979, report no. 559).
In all three tests, the concentrations were not analytically verified, but 3,3'-iminodi(propylamine) is expected to be stable in water, as the substance is miscible in water (Gestis database 2012), its vapor pressure is moderate (VP = 1.26 Pa @20°C, BASF AG 1987, report no. 187.0002.1) and its Henry's Law Constant is low (HLC = 5.54 E-08 Pa m³/mol @ 25°C, calculated with HENRYWIN v3.20, not in applicability domain, BASF SE 2017).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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