3,3'-iminodi(propylamine)

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented study which meets basic scientific principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

3,3'-iminodi(propylamine)

EC Number:

200-261-2

EC Name:

3,3'-iminodi(propylamine)

Cas Number:

56-18-8

Molecular formula:

C6H17N3

IUPAC Name:

bis(3-aminopropyl)amine

Details on test material:

- Name of test material (as cited in study report): Dipropylentriamin
- Analytical purity: 99%

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Firma WIGA (Sulzfeld)
- Weight at study initiation: 185 ± 15 gram
- Diet: Tieren Herilan MRH (Firma H . EGGERSMANN KG, Rinteln/Weser), ad libitum
- Water: ad libitum

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

nose/head only

Vehicle:

other: ethanol

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: a constant quantity was ensured by a continuous infusion pump (UNITA I, B. BROWN, Melsungen) and by means of compressed air in a two-material nozzle which produces aerosols.
- Source and rate of air: 1000 L/h

TEST ATMOSPHERE
- Brief description of analytical method used: Gaschromatograph HP 5040 A with a 2 m glass colum (2 mm i.d.), with a Carbowax 20 M separation phase and Supelcoport 80/100 mesh carrier. The oventemperature is 200 °C, the detector (FID) and injector temperature are 280 °C, the corriergas flow is 20.4 ml/in, the hydrogen flow rate is 30 ml/min and the air flow is 255 ml/min. The retetion time of the internal standard (C17KW) is 2.66 min and the retetion time of the sample is 5.06.
- Samples taken from breathing zone: yes

CLASS METHOD
- Rationale for the selection of the starting concentration: First orientating dose was: 2.5 mg/L (analytical determined)

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

Nominal concentrations: 0.015, 0.037, 0.063, 0.47, 0.931, 4.66 and 8.37 mg/L
Measured concentrations: 0.014, 0.027, 0.055, 0.235, 0.606, 1.3 and 2.0 mg/L

No. of animals per sex per dose:

10

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of weighing: before dosing, after 7 days and after 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight.

Statistics:

Probability analyses from D.J. Finney ( D.J. Finney; Probitanalysis 1971, Seite 1-150). The LC50 was calculated with 95% probability.

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LC50

Effect level:

ca. 0.03 mg/L air

Based on:

test mat.

Exp. duration:

4 h

Effect levels 2

Sex:

male

Dose descriptor:

LC50

Effect level:

0.04 mg/L air

Based on:

test mat.

Exp. duration:

4 h

Effect levels 3

Sex:

female

Dose descriptor:

LC50

Effect level:

0.03 mg/L air

Based on:

test mat.

Exp. duration:

4 h

Mortality:

0.014 mg/L: none of the animals died after 14 days
0.027 mg/L: 3/10 males and 6/10 females after 14 days
0.055 mg/L: 8/10 males and 9/10 females after 14 days
0.235 mg/L and up: all animals died after 14 days

Clinical signs:

other: Red eye and nasal secretion, dyspnoea, imbalanced and insecure walk, crouched position, apathy, bad general condition, ragged and clotty fur, 2/10 females suffered hair loss, shaking, delayed pain reaction, cyanosis.

Body weight:

0.014 mg/L: normal body weight gain
0.027 mg/L: reduced body weight gain
0.055 mg/L: body weight loss

Gross pathology:

Diseased animals:
Acute dilatation of the heart, acute congested hyperemia, notable wet fleshy, edematous and diffuse redness of the lungs(bloody, lung hemorrhages), peripheral lobular pattern in the liver.

Euthanized animals:
0.014 mg/L: no abnormal findings
0.027 mg/L: carnification of the lungs
0.055 mg/L: strong weight loss, areas of carnification in the lungs

Applicant's summary and conclusion

Executive summary:

In an inhalation toxicity study, comparable to OECD 403, Sprague-Dawley rats (10/sex/dose) were exposed (nose/head only) for 4 hours to 0.014 to 2.0 mg dipropylenetriamine aerosols/L air followed by an 14 day observation period. The LC50 was ca. 0.03 mg/L air.