Registration Dossier
Registration Dossier
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EC number: 201-873-2 | CAS number: 88-99-3
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Basic data given
- Qualifier:
- according to guideline
- Guideline:
- other: ISO 10712, 1995 Pseudomonas putida growth inhibition test
- Principles of method if other than guideline:
- Method: other: (Pseudomanas cell multiplication inhibition test)
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Test organisms (species):
- Pseudomonas putida
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 16 h
- Details on test conditions:
- Type: aquatic
- Duration:
- 16 h
- Dose descriptor:
- EC50
- Effect conc.:
- 213 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Executive summary:
The effect of phthalic acid towards Pseudomonas putida was investigated according to ISO 10712 having a 16 h-EC50 of 213 mg/l
(Sepic, 2003).
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- unsuitable test system
- Principles of method if other than guideline:
- Method: other: static toxicity test
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Test organisms (species):
- Vibrio fisheri
- Test type:
- static
- Water media type:
- saltwater
- Details on test conditions:
- Type: aquatic
- Duration:
- 15 min
- Dose descriptor:
- EC50
- Effect conc.:
- 6.61 mg/L
Referenceopen allclose all
The inhibition effect was determined by the increase of turbidity of bacterial cultures at chosen logarithmic dilutions of the initial concentration of the test substance
Ecotoxicological descriptors were the concentration values causing a 50 % inhibition of bioluminescence after 15 min exposure.
Description of key information
The effect of phthalic acid towards Pseudomonas putida was investigated according to ISO 10712 having a 16 h-EC50 of 213 mg/l (Sepic, 2003).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 213 mg/L
Additional information
There is only one reliable test with phthalic acid on microorganisms available. The toxicity of phthalic acid to Pseusomonas putida was tested in a 16 hours test with pH adjustment to 7.4. Population growth impairment was used as endpoint. The test was performed according to the ISO 10712 method. An EC50 of 213 mg/l was observed (Sepic, 2003).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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