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EC number: 407-560-9
CAS number: 107934-68-9
Measured concentrations of the test substance in the limit test
Mortalities and abnormalities in the limit test (10 fish in each
A study was performed to assess the acute toxicity of the test
item to fish using the species Gobiocypris rarus. The method followed
was designed to be compatible with the OECD Guidelines for Testing of
Chemicals No 203 also the Guidance document on aquatic toxicity testing
of difficult substances and mixtures, OECD series on testing and
assessment number 23, 2000 under GLP conditions.
Due to the low aqueous solubility and complex nature of the test
item, for the purposes of the test, the test item was prepared as a
Water Accommodated Fraction (WAF).Ten fish were exposed to a Water
Accommodated Fraction (WAF) of the test item at a single nominal loading
rate of 100 mg/L for 96 hours at a temperature of ca. 22 °C under
semi-static test conditions. The number of dead fish and any adverse
reactions to exposure were recorded after 3, 24, 48, 72 and 96 hours.
The 96 -Hour LL50 for fish based on nominal loading rates was
greater than 100 mg/L loading rate, equivalent to greater than 0.06 mg/L
(Time-weighted average) based on measured concentrations (LC50). This
indicates that, in the range of its water solubility, the test item was
not toxic under the test conditions.
LC50 (96h) > 0.06 mg/L (measured) for
Chinese rare minnow (OECD 203).
No toxicity observed at the limit of
solubility of the test substance.
The same observation was done in a 1991
study on Oncorhynchus mykiss (OECD 203) although a solubilizer was used
and so the fish were exposed to concentrations above the limit of
solubility of the test substance.
Two studies are available on the short-term
toxicity of 4,4´-(9H-fluoren-9-ylidene)bis(2-chloroaniline) (CAS No.
107934-68-9) to fish. Both studies were performed according to OECD
guideline 203 and GLP, with Chinese rare minnow or Rainbow trout as test
organism. The fish were exposed to the substance for 96 hours under
semi-static conditions. Both tests were conducted as limit tests with a
measured test substance concentration of 0.06 mg/L (Chinese rare minnow,
no solubilizer, loading rate 100 mg/L) and 6.5 mg/L (Rainbow trout,
solubilizer, loading rate 10 mg/L). During the 96 hours test duration no
effects on the test fish were observed.
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