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EC number: 252-552-9
CAS number: 35415-27-1
Details on results
Table 1: Dissolved oxygen concentrations (mg/L) in the closed bottles:
Oxygen concentration (mg/L)
Oc: Mineral nutrient solution with only inoculum.
Ocs: Mineral nutrient solution without test material but with inoculum
Ot: Mineral nutrient solution with test material (2.0 mg/L), surfactant,
Oa: Mineral nutrient solution with sodium acetate (6.7 mg/L) and with
Table 2: Oxygen consumption (mg/L) and the percentages biodegradation of
the test substance (BOD/ThOD) and sodium acetate (BOD/ThOD) in the
Closed Bottle test:
Oxygen consumption (mg/L)
Inhibition of the degradation of an easily-degradable compound, e.g.
sodium acetate by the test compound in the Closed Bottle test was not
determined because possible toxicity of the test substance to
microorganisms degrading acetate is not relevant. Inhibition can be
detected prior to the onset of the biodegradation through suppression of
the oxygen consumption in the presence of the test substance (higher
oxygen concentration in bottles with test substance compared to the
concentration in the control bottles). Inhibition of the endogenous
respiration of the inoculum by the test substance tested was not
detected (Table 1). Therefore, no inhibition of the biodegradation due
to the "high" initial concentration of the test item is expected.
The pH of the media was 8.0 at the start of the test. The pH of the
medium at day 28 was 7.9 (control with surfactant) and 8.0 (test and
control). Temperatures were within the prescribed temperature range of
22 to 24°C.
Validity of the test:
The validity of the test is demonstrated by an endogenous respiration of
1.1 mg/L at day 28 (Table 1). Furthermore, the differences of the
replicate values at day 28 were less than 20%. The biodegradation
percentage of the reference compound, sodium acetate, at day 14 was 76.
Finally, the validity of the test is shown with oxygen concentrations
remaining at >0.5 mg/L in all bottles over the test period.
In order to assess the biotic degradation, a ready biodegradability test
was performed which allows the biodegradability to be measured in an
aerobic aqueous medium. The ready biodegradability was determined in the
Closed Bottle test performed according to slightly modified OECD, EU and
ISO Test Guidelines, and in compliance with the OECD principles of Good
Laboratory Practice. River water was used as the inoculum.
Trinonyl benzene-1,2,4-tricarboxylate did not cause a reduction in the
endogenous respiration. The test substance is therefore considered to be
non-inhibitory to the inoculum. The test substance was biodegraded by
48% at day 28 in the Closed Bottle test.
In the prolonged Closed Bottle test, the test item was biodegraded by
63% at day 60 (enhanced biodegradability testing). This result
demonstrates that the test substance is inherently biodegradable.
Moreover, a biodegradation percentage of >60 within a 60-day time period
allows classification of trinonyl benzene-1,2,4-tricarboxylate as not
The test is valid as shown by an endogenous respiration of 1.1 mg/L and
by the total mineralization of the reference compound, sodium acetate.
Sodium acetate was degraded by 76% of its theoretical oxygen demand
after 14 days. Finally, the most important criterion was met with oxygen
concentrations remaining at >0.5 mg/L in all bottles over the test
The submission substance is not persistent: 63% biodegradation at day 60 (inoculum river water, aerobic test conditions).
According to these results on biodegradation, the submission substance
does not persist in the environment.
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