Trinonyl benzene-1,2,4-tricarboxylate

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2016-08-17 to 2016-10-24

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

performed compliant with GLP

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Version / remarks:

adopted July 1995

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

column elution method

Remarks:

slow stirring

Specific details on test material used for the study:

Test item: Trinonyl benzene-1,2,4-tricarboxylate
Batch No.: 50000071656
Identity: Trinonyltrimellitat
Molecular weight: 588.86 g/mol
Purity: 99.8 % (ester content)
Physical state: Clear colourless liquid
Storage conditions: Ambient temperature (10 °C to 30 °C)
Expiry date: August 2017
Certificate: Certificate of analysis dated August 08, 2016, OXEA GmbH, 46147 Oberhausen, Germany

Key result

Water solubility:

< 103 µg/L

Conc. based on:

test mat.

Remarks:

Concentration below limit of quantification (100 µg/L)

Temp.:

20 °C

pH:

6.5

Remarks on result:

other:

Remarks:

Concentrations of the test item in all fractions of both columns (12.5 ml/h; 25 ml/h) were below LOQ of 103 µg/L.

Details on results:

See section "Any otther information on results incl. tables"!

Column 1: Rotation period of the fraction collector: 90 min per fraction;

Duration of sampling: about 92 h (61 fractions):

Column elution method, column 1 (approximately 12.5 mL/h)

Fraction no.	V / ml	pH	water solubility /µg/l
35	15.5	6.5	< 103
36	15.5	6.6	< 103
37	15.5	6.6	< 103
38	15.5	6.6	< 103
39	15.5	6.5	< 103

The water solubility was found to be below the quantification limit of 103 µg/L.

Column 2: Rotation period of the fraction collector: 45 min per fraction;

Duration of sampling: about 92 h (122 fractions):

Column elution method, column 2 (approximately 25.0 mL/h)

Fraction no.	V / ml	pH	water solubility /µg/l
70	12.5	6.5	< 103
71	12.0	6.6	< 103
72	12.0	6.6	< 103
73	12.0	6.5	< 103
74	12.0	6.5	< 103

The water solubility was found to be below the quantification limit of 103 µg/L. In both columns the concentration of five consecutive fractions was constant within ±30 % No increasing or decreasing tendency was observed.

Coating check:

The coating check was successful. Enough test item remained on the columns:

	Amount of test item used for coating / mg	Measured concentration of test item in eluate / mg/l	Amount of test item eluted / mg
Column 1	27.5	0.460 / 0.451	45.5^1)
Column 2	27.5	0.213 / 0.219	21.6

1) After the sampling period a higher amount of test item was found on column 1 than used for coating. This could have been caused by the addition of twice the initial weight at the beginning of the test. This deviation had no influence on the final result.

Conclusions:

Water solubility (OECD 105; EU-method A.6): < 0.1 mg/L (20°C)

Executive summary:

The water solubility of the submission substance was determined according to OECD 105 and EU-method A.6 compliant with GLP. Due to the low water solubility determined in preliminary testing foreseen according to the test guidelines, the column elution method was applied using sea sand as the inert solid support. Test item concentrations within all elution fractions of both column elution experiments (flow rates 12.5 mL/h and 25 mL/h) were below the limit of quantification (103 µg/L). At the end of the experiment, for both columns a "coating check" was performed to demonstrate that still sufficient test item material was bound on the sea sand. This could be confirmed by elution with acetone (>78% of initial loading recovered).

In conclusion, the water solubility of the test item as determined via the column elution method at 20°C was found to be below the quantification limit of the analytical method:

water solubility (20°C) < 0.1 mg/L

Description of key information

 Water solubility (OECD 105; EU-method A.6): < 0.1 mg/L (20°C)

Key value for chemical safety assessment

Water solubility:

100 µg/L

at the temperature of:

20 °C

Additional information

The water solubility of the submission substance was determined according to OECD 105 and EU-method A.6 compliant with GLP. Due to the low water solubility determined in preliminary testing foreseen according to the test guidelines, the column elution method was applied using sea sand as the inert solid support. Test item concentrations within all elution fractions of both column elution experiments (flow rates 12.5 mL/h and 25 mL/h) were below the limit of quantification (103 µg/L). At the end of the experiment, for both columns a "coating check" was performed to demonstrate that still sufficient test item material was bound on the sea sand. This could be confirmed by elution with acetone (>78% of initial loading recovered).

In conclusion, the water solubility of the test item as determined via the column elution method at 20°C was found to be below the quantification limit of the analytical method:

Water solubility (20°C) < 0.1 mg/L