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EC number: 252-552-9
CAS number: 35415-27-1
Results growth inhibition of Selenastrum capricornutum exposed to the
Toxicity to green algae (Selenastrum capricornutum) was investigated
during 72 hours of exposure according to OECD 201 (adopted 1984) and in
compliance with GLP. As the test substance is of low water solubility, a
dispersion agent was used to increase the test concentration in the test
medium (hydrogenated castor oil HCO-50, 100 mg/L). The test was
performed using seven test concentrations (nominal 0.79 mg/L, 1.57 mg/L,
3.13 mg/L, 6.25 mg/L, 12.5 mg/L, 25.0 mg/L and 50 mg/L). A blank and
vehicle control were run in parallel.
During 72 hours, the cell density increased by a factor of 132 for the
blank and a factor by 129 for the dispersant control. For the lowest
test concentration, the cell density increased by a factor of 128 and
was similar compared to the controls. The cell density increased by a
factor of 108 for the highest test concentration indicating a slight
inhibition of growth. For the other five concentrations (1.57 mg/L, 3.13
mg/L, 6.25 mg/L, 12.5 mg/L, 25.0 mg/L), the cell density increased by a
factor of 111 – 122.
Actual measurements of the test concentration were within the range of ±
20% of the nominal concentration, and therefore effect concentrations
were based on the nominal test concentration. The 72h-EbC50 (green
algae, biomass) was > 50.0 mg/L and the 72h-NOECb was > 50.0 mg/L; the
72hErC50 (green algae, growth rate) was > 50.0 mg/L and the 72h-NOECr
was 25.0 mg/L.
The validity criteria of the test guideline are fulfilled (OECD 201
adopted 1984) and results of the study are considered reliable. With
regard to the validity criteria of the updated guideline (2006), the
coefficients of variation for the section-by-section-growth rates of the
controls and average specific growth rates were newly calculated (as far
as available data allowed the assessment) and are below the permitted
maximum value. Therefore, the study also meets the validity criteria of
the updated guideline.
No aquatic toxicity data on plants for the submission substance is available. However adequate and reliable data for structural analogues is available (i.e. C8TM and C8C10TM, for read-across justification please see read-across report in section 13). The derived 72h-NOECr (green algae, growth rate) = 27.0 mg/L (corrected for the molecular weight of the target substance).
No aquatic toxicity data for the submission substance is available.
However adequate and reliable data for structural analogues (i.e. C8TM
and C8C10TM, for read-across justification please see read-across report
in section 13) are reported below.
In a reliable long-term toxicity study on green algae (according to OECD
201 and in compliance with GLP, MoE Japan, 2001, RL1), effects on the
growth rate were observed after 72 hours of exposure, but not on yield
of biomass (analogue substance: trioctyl benzene-1,2,4-tricarboxylate,
trioctyl ester, C8TM, CAS no. 89-04-3). The tested concentrations lay
above the water solubility of the test substance, except for the lowest
of measured 0.79 mg/L (solubilizer was used). No significant adverse
effects were observed in concentrations up to 25 mg/L (corresponding to
27 mg/L of the target substance). Test concentrations of the supporting
study lay below the water solubility (lowest at nominal 0.24 mg/L), and
no effects were observed up to the highest test concentration of 3.5
mg/L (OECD 201, GLP, Huels AG, 1994, RL2; analogue substance:
1,2,4-benzenetricarboxylic acid, mixed decyl and octyl triesters,
C8C10TM, CAS no. 90218-76-1; corresponding to 3.3 -3.8 mg/L of the
target substance). In conclusion, no toxicity to green algae is expected
below the water solubility of the analogue and submission substance.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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