Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 310-290-3
CAS number: 161907-80-8
NOEC = 1000 mg/L
An experimental study for the determination
of the toxicity of B-TTEGME to microorganisms is not available. QSAR
analysis was not performed since "the use of QSARs for predicting STP
toxicity is still limited. Although there are some QSARs for toxicity to
microorganisms published...., this is not a very well developed science
domain today." (Guidance on information requirements and chemical safety
assessment, Chapter R.7B: Endpoint specific guidance, section
R.184.108.40.206, page 136). However, there is sufficient information from
read across and a study with a B-TTEGME containing Brake Fluid to
perform the risk assessment as outlined below.
B-TTEGME is mixture of different borated
glycol esters. The length of the glycol ester moiety ranges from 3 to 5
glycols per glycol ester moiety. About 50 % of B-TTEGME have a chain
length of 3 glycols per glycol ester moiety. The molecule with the chain
length of 3 glycol esters is registered under the name B-TEGME (cas#
30989 -05 -0). Therefore, B-TEGME is relevant for the risk assessment of
Following information is available for
the GLP compliant study from BASF (1999) the
effect of B-TEGME on the oxygen consumption of activated sludge was
determined in a limit test with 1000 mg B-TEGME/L. The study is
considered compliant with OECD 209, ISO 8192 and C.11. The inoculum was
obtained from laboratory wastewater plants treating municipal waste
water. The inoculum was washed, adjusted to 5g dry mass/L and
subsequently aerated for 24 hours. Three controls and one replicate for
the treated test solution was set up. Non-replicated positive controls
were set up with 1, 10 and 100 mg 3,5 -dichlorophenol/L The respiration
measurements were performed 30 minutes after start of the exposure.
test with 3,5 -dichlorophenol resulted in an EC50 of 8.8 mg 3,5
-dichlorophenol/L. The variation between 3 controls was < 15%. The
findings from the positive controls render the test as valid. At 1000 mg
B-TEGME/L, the respiration was increased by 6% relative to the average
respiration in the control. Therefore, the EC0 is 1000 mg B-TEGME/L and
the EC20, EC50 and EC80 are > 1000 mg B-TEGME/L and consequently B-TEGME
is considered to have no effect on microorganisms.
the GLP compliant study from Shell (1986), the microbial inhibition of a
brake fluid (which contains 35% B-TTEGME) to Pseudomonas fluorescens was
determined in a microbial inhibition test. The test design was based on
the method described in "Degradability, Ecotoxicity, and
Bioaccumulation, TNO, Delft, The Netherlands, 1977". The nominal test
concentrations were 0 (Control), 10, 32, 100, 320, and 1000 mg brake
fluid/L (nominal concentrations), corresponding to 0 (control), 35, 11.2,
35, 112, and 350 mg B-TTEGME/L, respectively. Sodium pentachlorophenate
was used as positive control. Growth curves were constructed of the
optical density of the inoculated media versus time and the rate
determined as the slope of the exponential growth phase. The reference
substance, sodium pentachlorophenate inhibited with an EC50 =18 mg/L
while the brake fluid caused a maximum of 19% inhibition at 1000 mg
brake fluid/L, corresponding to 350 mg B-TTEGME/L, the highest
concentration tested. Therefore, the EC50 is > 350 mg B-TTEGME/L. The
exposure duration, environmental conditions like temperature and details
on the measurements of the optical density and the origin of the
cultures were not reported. Due to the limited information provided the
results of this study are considered to have only limited relevance and
the study was considered to be supporting.
GLP study with B-TEGME (BASF, 1999) provided an EC10 and EC50 of >
1000 mg B-TEGME/L. The advantage of this study is that it was performed
with B-TEGME itself and not with a B-TTEGME containing brake fluid as in
the study from Shell
other additives may affect microbial growth. The (supporting) study from
an EC50 of > 395 mg B-TTEGME/L, the highest tested concentration. Due to
the above restrictions the test results from Shell
not considered as relevant for the risk assessment. In
conclusion, the EC10 and EC50 values of >1000 mg B-TEGME /L are
considered reliable and sufficiently conservative to assess the toxicity
of B-TEGME on microorganisms.
For following reasons the study from BASF
(1999) on B-TEGME is considered to give sufficient indication for the
assessment of the toxicity of B-TTEGME to microorganisms:
on the above considerations, there is no indication that B-TTEGME may be
more toxic to microorganisms than B-TEGME. Consequently, a NOEC of 1000
mg B-TTEGME/L is used for
the risk assessment of B-TTEGME.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Ce site web utilise des cookies afin de vous garantir la meilleure expérience possible sur nos sites web.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again