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EC number: 310-290-3
CAS number: 161907-80-8
EC50 >422.4 mg/L acute Daphnia magna immobilization (OECD 202)
the assessment of the EC50 for freshwater invertebrates, two acute Daphnia
studies are available, one key study with a mixture of methyl borate
esters which contains 44% B-TTEGME (Shell 1999) and one supporting study
with a brake fluid containing 35% B-TTEGME (Shell 1987).
the GLP compliant (key) study from Shell (1999) the acute toxicity of
methyl borate esters (containing 44% B-TTEGME) to Daphnia magna was
determined in a static 48 h toxicity limit test. The test was performed
according to OECD 202. No
relevant restrictions appeared and the only disadvantage of this study
is the fact that the study was performed with a B-TTEGME containing
mixture and not with B-TTEGME as pure substance itself.
The nominal test concentrations were 0 (control) and 1000 mg methyl
borate esters/L, corresponding to 0 (control) and 440 mg B-TTEGME/L. The
mean measured concentration was 960 mg methyl borate esters/L,
corresponding to 422.4 mg B-TTEGME/L. The control and the treated test
group was started with 2 replicates (10 daphnids each). Within the first
24 hours of exposure, 0 and 4 out of 20 daphnids were immobilized in the
control and at 422.4 mg B-TTEGME/L, respectively. Thereafter, no further
immobilization was observed. The 24 and 48 -hour EC50 were both >422.4
the GLP compliant (supporting) study from Shell (1987) the acute
toxicity of a brake fluid (which contains 35% B-TTEGME) to Daphnia
magna was determined in a static 48 h toxicity test. The test design
was similar to OECD 202. Restrictions of this study are (1) no specific
guideline was followed (2) dose verification analysis was not performed
(3) the temperature of 18- 22°C exceeded the maximum allowable variation
of +/- 1°C as given in the OECD 202 test guideline. Furthermore, the
test substance was a mixture of B-TTEGME and other components. The
nominal test concentrations were 0 (control), 7, 17.5, 35, 70, 175, and
350 mg B-TTEGME/L, applied as 0 (control), 20, 50, 100, 200, 500, and
1000 mg brake fluid /L. For the control and each of the treated test
groups three replicates with 10 daphnids younger than 24 hours were set
up. Within the 48 hour exposure period, 1 out of 30 daphnids was
immobilized in the 35, 70, and 350 mg B-TTEGME/L test groups. The 24 and
48 -hour EC50 values were both >350 mg B-TTEGME/L.
key study of Shell (1999) shows an EC50 of >422.4
mg B-TTEGME/L The supporting study of Shell (1987) provides an EC50
value of > 350 mg B-TTEGME/L. Due to the above given restrictions, this
value is considered less reliable than the value provided by Shell
conclusion, the EC50 value of 422.4 mg B-TTEGME/L is considered reliable
and sufficiently conservative to assess the toxicity of B-TTEGME on
above value is higher than the limit for classification and labeling, i.
e., higher than 100 mg B-TTEGME/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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