Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 207-236-5
CAS number: 455-14-1
Effects of TFMA isomers on the blood cell counts and on enzyme activities:
4-TFMA (in a dose of 0.66 mmol/kg of body weight) strongly increased the total number of leukocytes 72 h after administration; all white tell types
were significantly (P < 0.01) involved in this change. Simultaneously a significant decrease in the number of erythrocytes as well as hemoglobin
concentration was recorded (P < 0.01-0.001). The same dose of 3-TFMA caused weaker, but still significant changes in red blood cells and in the
number of leukocytes and lymphocytes (P < 0.05-0.001). Small changes in peripheral blood cell counts evoked by the administration of 2-TFMA
were not statistically significant.
Besides a great increase of TdK activity in spleen, caused also by the 3-TFMA administration, remarkable changes in activities of studied enzymes
occurred only after 4-TFMA administration. A suppression of ADA activity in both organs as well as of TdK activity in thymus and IP activity in spleen
Dose dependence of 4-TFMA effect:
Strong dose dependence was observed after 4-TFMA administration. Leukocytosis and especially lymphocytosis occurred at a starting dose of
0.165 mmol/kg of 4-TFMA. The highest dose in our experiment (0.66 mmol/kg), caused both the highest increase in the number of white blood
cells (P < 0.001) as well as the highest injury of red blood cells (anisocytosis, poikilocytosis, polychromasia). In both organs only weak dose
dependence of enzyme activities was recorded. Increase in TdK activity in the spleen and IP activity in the thymus intensified in the whole range
of doses used, as well as a mild decrease in ADA and IP activity in the spleen. In the thymus, decrease of TdK and ADA activity was found only
after the administration of the higher dose of the drug.
Weight of organs:
The splenomegaly induced by the single dose administration of 4-TFMA occurred in a dose-dependent manner, except for the highest dose
(0.66 mmol/kg), which seemed to give toxic effects. In the thymus, the highest dose of the drug induced maximum decrease in thymus weight.
Time-dependent decrease of thymus weight culminated 72 h after the administration of 4-TFMA (0.33 mmol/kg), while the highest weight of
spleen was found 72 h later.
Administration of a single dose of 4-TFMA (0.66 mmol/kg) resulted in a significant increase in the total amount of leukocytes as well as all
respective cell types in peripheral blood (P < 0.05-0.001). This effect culminated 48 h after administration of the drug and partially persisted
even after 144 h. A significant depression of the number of erythrocytes and concentration of hemoglobin (P < 0.05-0.001) persisted between 4 and 144 h after the administration of 4-TFMA.
Enzyme activity of TdK was considerably increased in spleen even 216 h after drug administration, as well as the lasting decrease of ADA and IP
activity. In thymus, continued inhibition of TdK and ADA activity was found, while activity of IP mildly increased between 6 and 72 h.
Histological changes in lyrnphatic organs after 4-TFMA administration:
Histological examination of thymus, spleen, lymphatic nodes and bone marrow was negative prior to application of 4-TFMA. Twenty-four hours
after administration of the drug (0.66 mmol/kg) a considerable decrease in the number of mature lymphocytes was observed in the thymus cortex
while in the medulla an increase in the number of lymphocytes occurred especially in the perivascular region. In the white pulp of the spleen, a
diminution of perivascular lymphatic sheaths and the majority of mature elements was found in their central zones. An enhanced lymphopoeisis
was detected in the peripheral region of perivascular sheaths. All animals (n = 4) exerted sinus hyperemia in the red pulp of the spleen and an
enhancement of lymphopoesis in Billroth cords.
In the lymphatic nodes an enlargement of cortical lymphatic machinery was followed by enhancement of lymphopoesis and an increase in
the number of centroblasts and centrocytes. A marked decrease in the number of lymphocytes was observed in paracortex and medulla.
Erythropoesis characterized by a slight increase in premature polychrome formps as well as increased lympho- and myelopoesis manifested
itself in bone marrow of investigated animals. No more histological changes were found in any of the tested organs 48 and 72 h after the
administration of 4-TFMA.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again