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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
N/A to 1983-07-14
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study was conducted with methods similar to OECD 401. However, information on the test substance was lacking (i.e., purity).
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1983
Report date:
1983

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Deviations:
yes
Remarks:
Information on the test substance (i.e., purity) was lacking.
Principles of method if other than guideline:
N/A
GLP compliance:
not specified
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl 3-mercaptopropionate
EC Number:
220-912-4
EC Name:
Methyl 3-mercaptopropionate
Cas Number:
2935-90-2
Molecular formula:
C4H8O2S
IUPAC Name:
methyl 3-sulfanylpropanoate
Details on test material:
- Name of test material (as cited in study report): methyl 3-mercaptopropionate
- Molecular formula (if other than submission substance): N/A
- Molecular weight (if other than submission substance): N/A
- Smiles notation (if other than submission substance): N/A
- InChl (if other than submission substance): N/A
- Structural formula attached as image file (if other than submission substance): N/A
- Substance type: active
- Physical state: liquid

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: N/A
- Age at study initiation: young adult
- Weight at study initiation: 203-288 g
- Fasting period before study: Animals were fasted 18 to 24 hours prior to dosing.
- Housing: Animals were housed two per cage.
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: at least one week


ENVIRONMENTAL CONDITIONS
- Temperature (deg. C): Every attempt was made to maintain temperatures at 70+/-4 deg. F.
- Humidity (%): Every attempt was made to maintain a relative humidity of 40-60 %.
- Air changes (per hr): N/A
- Photoperiod (hrs dark / hrs light): 12/12


IN-LIFE DATES: From: N/A To: N/A

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
unchanged (no vehicle)
Details on oral exposure:
VEHICLE
- Concentration in vehicle: N/A
- Amount of vehicle (if gavage): N/A
- Justification for choice of vehicle: N/A
- Lot/batch no. (if required): N/A
- Purity: N/A


MAXIMUM DOSE VOLUME APPLIED: Dosage volume for each rat was based on initial body weights.


DOSAGE PREPARATION (if unusual): N/A


CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: N/A
Doses:
50, 100, 150, 250, 500 and 5000 mg/kg bw
No. of animals per sex per dose:
5/sex/dose
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Observations performed on day of dosing (1, 2 and 4 hours) and twice daily thereafter. Observations included nature, onset, severity and duration of pharmacotoxic signs. Body weights were recorded at study initiation, day 7 and at termination (day 14).
- Necropsy of survivors performed: yes
- Other examinations performed: gross pathology
Statistics:
LD50 calculations were estimated by the Behrens-Reed-Meunch method.

Results and discussion

Preliminary study:
N/A
Effect levelsopen allclose all
Sex:
male
Dose descriptor:
LD50
Effect level:
206.4 mg/kg bw
95% CL:
149 - 285.9
Remarks on result:
other: See mortality section below.
Sex:
female
Dose descriptor:
LD50
Effect level:
181.7 mg/kg bw
95% CL:
132.6 - 248.9
Remarks on result:
other: See mortality section below.
Sex:
male/female
Dose descriptor:
LD50
Effect level:
193.6 mg/kg bw
95% CL:
154.2 - 243.2
Remarks on result:
other: See mortality section below.
Mortality:
Males:
No animals died in the groups exposed to 50-150 mg/kg of the test substance. 4 animals in the 250 mg/kg dose group died (within 2 hours of treatment) and all of the animals in the 500 and 5000 mg/kg dose groups died (within 1 hour of treatment).

Females:
One animal died (within 1 hour of treatment) in the 150 mg/kg dose group. All animals died (within 2 hours of treatment) in the dose groups of 250-5000 mg/kg.
Clinical signs:
other: Clinical observations were noted among all rats during the study. All rats dosed at 50 mg/kg bw exhibited slight depression on Day 1 post-dose only. Clinical signs noted in animals of remaining dose levels included one or more of the following: depressi
Gross pathology:
There were no gross pathological findings noted in rats surviving to termination. Findings among the rats that were found dead included dark red lungs, test substance-like material in the stomach and/or intestines, and reddish fluid in the intestines.
Other findings:
- Organ weights: N/A
- Histopathology: N/A
- Potential target organs: N/A
- Other observations: N/A

Any other information on results incl. tables

N/A

Applicant's summary and conclusion

Interpretation of results:
Category 3 based on GHS criteria
Remarks:
Migrated information
Conclusions:
The acute oral LD50 in male and female rats was determined to be 193.6 mg/kg bw.
Executive summary:

N/A