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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1999
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP study conducted according to OECD Guideline 202.
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
not specified
Principles of method if other than guideline:
N/A
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl 3-mercaptopropionate
EC Number:
220-912-4
EC Name:
Methyl 3-mercaptopropionate
Cas Number:
2935-90-2
Molecular formula:
C4H8O2S
IUPAC Name:
methyl 3-sulfanylpropanoate
Details on test material:
- Name of test material (as cited in study report): methyl 3-mercaptopropionate
- Molecular formula (if other than submission substance): Not applicable
- Molecular weight (if other than submission substance): Not applicable
- Smiles notation (if other than submission substance): Not applicable
- Substance type: active
- Physical state: liquid
- Analytical purity: 99.75%
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
N/A

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
N/A

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
-Method: A stock solution was prepared before the beginning of the test, by mixing 100 mg of the test substance with 1 liter of dilution water. Stock was then diluted to the desired concentrations.
- Eluate: N/A
- Differential loading: N/A
- Controls: Dilution water only
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): N/A
- Concentration of vehicle in test medium (stock solution and final test solution): N/A
- Evidence of undissolved material (e.g. precipitate, surface film, etc): N/A

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Strain: Straus Clone A
- Source: In house culture
- Age at study initiation (mean and range, SD): <24 h
- Weight at study initiation (mean and range, SD): N/A
- Length at study initiation (length definition, mean, range and SD): N/A
- Valve height at study initiation, for shell deposition study (mean and range, SD): N/A
- Peripheral shell growth removed prior to test initiation: N/A
- Method of breeding: parthanogenic
- Feeding during test
- Food type: N/A
- Amount: N/A
- Frequency: not fed during test


ACCLIMATION
- Acclimation period: N/A
- Acclimation conditions (same as test or not): N/A
- Type and amount of food: N/A
- Feeding frequency: N/A
- Health during acclimation (any mortality observed): N/A


QUARANTINE (wild caught)
- Duration: N/A
- Health/mortality: N/A

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
N/A

Test conditions

Hardness:
No data
Test temperature:
20-21 deg. C
pH:
7.75-7.93
Dissolved oxygen:
7.9-8.4 mg/L. Media aerated to saturation prior to test.
Salinity:
N/A
Nominal and measured concentrations:
Nominal- Control, 0.06, 0.13, 0.28, 0.62, 1.36, and 3.00 mg/L.
Measured concentrations were provided in Table 1.
Details on test conditions:
TEST SYSTEM
- Test vessel: 120 mL Erlenmeyer
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: PTFE and aluminum caps
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): N/A
- Renewal rate of test solution (frequency/flow rate): N/A
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): N/A
- Biomass loading rate: N/A


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: -Dilution water is prepared in the laboratory using pure water and salts according to ISO 6341.

25 mL/L of the solutions below, aerated up to oxygen saturation:
11.76 g CaCl2, 2 H2O /L ultrapure water
4.93 g MgSO4, 7 H2O /L ultrapure water
2.59 g NaHCO3 /L ultrapure water
0.23 g KCl /L ultrapure water

- Total organic carbon:
- Particulate matter: N/A
- Metals: N/A
- Pesticides: N/A
- Chlorine: N/A
- Alkalinity: N/A
- Ca/mg ratio: 4
- Conductivity: N/A
- Culture medium different from test medium: N/A
- Intervals of water quality measurement: N/A


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: test conducted in the dark
- Light intensity: N/A


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobilization, observed at 24 h intervals.


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Justification for using less concentrations than requested by guideline: N/A
- Range finding study
- Test concentrations: N/A
- Results used to determine the conditions for the definitive study:N/A
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.55 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
N/A
Results with reference substance (positive control):
N/A
Reported statistics and error estimates:
N/A

Any other information on results incl. tables

 

 

Table 1. Nominal vs. Measured Concentrations

Nominal Conc.

Measured concentrations (mg/L)

Initial

Final

Final/Initial (%)

0.06

NA

NA

-

0.13

NA

NA

-

0.28

0.279

0.258

92.47

0.62

0.604

0.590

97.68

1.36

1.275

1.236

96.94

3.00

2.763

2.719

98.41

NA-not analyzed

 

Table 2. Percent Immobilization over Time

Nominal conc. (mg/L)

% Immobilization

24h

48h

Control

5

10

0.06

0

5

0.13

0

0

0.28

20

45

0.62

55

85

1.36

45

85

3.00

35

90

 

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 48h EC50 of the test substance to D.magna is 0.55 mg/L.
Executive summary:

N/A