Methyl 3-mercaptopropionate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study conducted according to OECD Guideline 202.

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

Principles of method if other than guideline:

N/A

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Methyl 3-mercaptopropionate

EC Number:

220-912-4

EC Name:

Methyl 3-mercaptopropionate

Cas Number:

2935-90-2

Molecular formula:

C4H8O2S

IUPAC Name:

methyl 3-sulfanylpropanoate

Details on test material:

- Name of test material (as cited in study report): methyl 3-mercaptopropionate
- Molecular formula (if other than submission substance): Not applicable
- Molecular weight (if other than submission substance): Not applicable
- Smiles notation (if other than submission substance): Not applicable
- Substance type: active
- Physical state: liquid
- Analytical purity: 99.75%

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
N/A

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

N/A

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
-Method: A stock solution was prepared before the beginning of the test, by mixing 100 mg of the test substance with 1 liter of dilution water. Stock was then diluted to the desired concentrations.
- Eluate: N/A
- Differential loading: N/A
- Controls: Dilution water only
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): N/A
- Concentration of vehicle in test medium (stock solution and final test solution): N/A
- Evidence of undissolved material (e.g. precipitate, surface film, etc): N/A

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain: Straus Clone A
- Source: In house culture
- Age at study initiation (mean and range, SD): <24 h
- Weight at study initiation (mean and range, SD): N/A
- Length at study initiation (length definition, mean, range and SD): N/A
- Valve height at study initiation, for shell deposition study (mean and range, SD): N/A
- Peripheral shell growth removed prior to test initiation: N/A
- Method of breeding: parthanogenic
- Feeding during test
- Food type: N/A
- Amount: N/A
- Frequency: not fed during test


ACCLIMATION
- Acclimation period: N/A
- Acclimation conditions (same as test or not): N/A
- Type and amount of food: N/A
- Feeding frequency: N/A
- Health during acclimation (any mortality observed): N/A


QUARANTINE (wild caught)
- Duration: N/A
- Health/mortality: N/A

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

N/A

Test conditions

Hardness:

No data

Test temperature:

20-21 deg. C

pH:

7.75-7.93

Dissolved oxygen:

7.9-8.4 mg/L. Media aerated to saturation prior to test.

Salinity:

N/A

Nominal and measured concentrations:

Nominal- Control, 0.06, 0.13, 0.28, 0.62, 1.36, and 3.00 mg/L.
Measured concentrations were provided in Table 1.

Details on test conditions:

TEST SYSTEM
- Test vessel: 120 mL Erlenmeyer
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: PTFE and aluminum caps
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): N/A
- Renewal rate of test solution (frequency/flow rate): N/A
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): N/A
- Biomass loading rate: N/A


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: -Dilution water is prepared in the laboratory using pure water and salts according to ISO 6341.

25 mL/L of the solutions below, aerated up to oxygen saturation:
11.76 g CaCl2, 2 H2O /L ultrapure water
4.93 g MgSO4, 7 H2O /L ultrapure water
2.59 g NaHCO3 /L ultrapure water
0.23 g KCl /L ultrapure water

- Total organic carbon:
- Particulate matter: N/A
- Metals: N/A
- Pesticides: N/A
- Chlorine: N/A
- Alkalinity: N/A
- Ca/mg ratio: 4
- Conductivity: N/A
- Culture medium different from test medium: N/A
- Intervals of water quality measurement: N/A


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: test conducted in the dark
- Light intensity: N/A


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobilization, observed at 24 h intervals.


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Justification for using less concentrations than requested by guideline: N/A
- Range finding study
- Test concentrations: N/A
- Results used to determine the conditions for the definitive study:N/A

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.55 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

N/A

Results with reference substance (positive control):

N/A

Reported statistics and error estimates:

N/A

Any other information on results incl. tables

 

 

Table 1. Nominal vs. Measured Concentrations

Nominal Conc.	Measured concentrations (mg/L)
	Initial	Final	Final/Initial (%)
0.06	NA	NA	-
0.13	NA	NA	-
0.28	0.279	0.258	92.47
0.62	0.604	0.590	97.68
1.36	1.275	1.236	96.94
3.00	2.763	2.719	98.41

NA-not analyzed

 

Table 2. Percent Immobilization over Time

Nominal conc. (mg/L)	% Immobilization
	24h	48h
Control	5	10
0.06	0	5
0.13	0	0
0.28	20	45
0.62	55	85
1.36	45	85
3.00	35	90

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48h EC50 of the test substance to D.magna is 0.55 mg/L.

Executive summary:

N/A