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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1997
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP study conducted according to OECD Guideline 301 B "Ready Biodegradability: Modified Sturm Test (CO2 evolution)"
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1997

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Deviations:
no
Principles of method if other than guideline:
N/A
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl 3-mercaptopropionate
EC Number:
220-912-4
EC Name:
Methyl 3-mercaptopropionate
Cas Number:
2935-90-2
Molecular formula:
C4H8O2S
IUPAC Name:
methyl 3-sulfanylpropanoate
Details on test material:
- Name of test material (as cited in study report): methyl 3-mercaptopropionate
- Molecular formula (if other than submission substance): N/A
- Molecular weight (if other than submission substance): N/A
- Smiles notation (if other than submission substance): N/A
- Substance type: active
- Physical state: liquid
- Analytical purity: 99.75%
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
N/A

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
other: secondary effluent from a biologic treatment plant
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): : 2.6*E5 bacteria/mL medium
secondary effluent from a biologic treatment plant of Versailles, France.
- Laboratory culture: N/A
- Method of cultivation: N/A
- Storage conditions: N/A
- Storage length: N/A
- Preparation of inoculum for exposure: N/A
- Pretreatment: N/A
- Concentration of sludge: N/A
- Initial cell/biomass concentration: 2.6*E5 bacteria/mL
- Water filtered: N/A
- Type and size of filter used, if any: N/A
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
37.5 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST CONDITIONS
- Composition of medium: N/A

- Additional substrate: N/A
- Solubilising agent (type and concentration if used): N/A
- Test temperature: 22 deg. C
- pH: N/A
- pH adjusted: yes/no N/A
- CEC (meq/100 g): N/A
- Aeration of dilution water: N/A
- Suspended solids concentration: N/A
- Continuous darkness: yes/no: N/A
- Other: N/A


TEST SYSTEM
- Culturing apparatus: N/A
- Number of culture flasks/concentration: N/A
- Method used to create aerobic conditions: N/A
- Method used to create anaerobic conditions: N/A
- Measuring equipment: N/A
- Test performed in closed vessels due to significant volatility of test substance: N/A
- Test performed in open system: N/A
- Details of trap for CO2 and volatile organics if used: N/A
- Other: N/A


SAMPLING
- Sampling frequency: 0, 1, 5, 8, 14, 20, 27, 28 days
- Sampling method: N/A
- Sterility check if applicable: N/A
- Sample storage before analysis: N/A
- Other: N/A


CONTROL AND BLANK SYSTEM
- Inoculum blank: N/A
- Abiotic sterile control: N/A
- Toxicity control: N/A
- Other: Appropriate controls and blank system were used. Details were not available.


STATISTICAL METHODS: N/A
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

Preliminary study:
N/A
Test performance:
- 46% after 28 days
- 21% degradation in the 10 days window
- No inhibition of inoculum observed.
% Degradation
Parameter:
% degradation (CO2 evolution)
Value:
46
Sampling time:
28 d
Details on results:
N/A

BOD5 / COD results

Results with reference substance:
Please see Tables 1-3 below.

Any other information on results incl. tables

Table 1. CO2 produced (mg/day)

Test Day

0

1

5

8

14

20

27

28

Blank 1

0

2.1

3.1

2.5

3.2

3.2

2.9

0.8

Blank 2

0

2.3

9.5

2.4

3.0

1.9

2.2

0.4

Mean

 

 

 

 

 

 

 

 

Blank

0

2.2

6.3

2.5

3.1

2.1

2.5

0.6

Test 1

0

2.1

3.1

6.8

6.9

5.3

13.1

0.6

Test 2

0

1.8

4.4

0.6

6.4

5.4

18.4

0.9

Mean

 

 

 

 

 

 

 

 

Test

0

1.9

3.7

3.7

6.7

5.3

15.7

0.8

Ref substance

0

33.2

35.3

2.6

2.2

1.0

0.2

0.2

Inhibition

0

14.5

16.1

13.5

11.4

7.9

0.2

-0.1

Sterile

0

2.2

1.3

0.4

0.1

-1.4

0.3

0.2

Reference substance is Sodium Benzoate.

Table 2. Cumulative CO2 produced (mg)

Test Day

0

1

5

8

14

20

27

28

Blank 1

0

2.1

5.2

7.7

10.9

13.2

16.1

16.8

Blank 2

0

2.3

11.8

14.2

17.2

19.0

21.2

21.7

Mean

 

 

 

 

 

 

 

 

Blank

0

2.2

8.5

10.9

14.0

16.1

18.6

19.3

Test 1

0

2.1

5.2

11.9

18.9

24.1

37.2

37.8

Test 2

0

1.8

6.2

6.8

13.1

18.5

36.9

37.8

Mean

 

 

 

 

 

 

 

 

Test

0

1.9

5.7

9.3

16.0

23.1

37.1

37.8

Ref substance

0

33.2

68.5

71.1

73.3

74.3

74.5

74.7

Inhibition

0

14.5

30.6

44.1

55.5

63.4

63.6

63.6

Sterile

0

2.2

3.5

4.0

4.1

2.6

3.0

3.2

Reference substance is Sodium Benzoate.

Table 3. Degradation (%)

Test Day

0

1

5

8

14

20

27

28

Test 1

0

3

6

14

23

29

45

46

Test 2

0

2

7

8

16

22

45

46

Mean

 

 

 

 

 

 

 

 

Test

0

2

7

11

19

26

45

46

           

Ref substance

0

40

83

86

89

90

90

91

Inhibition

0

9

19

27

34

38

39

39

Sterile

0

3

4

5

5

3

4

4

Reference substance is Sodium Benzoate.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
other: not readily biodegradable
Conclusions:
46% degradation in 28 days; not readily biodegradable.
Executive summary:

N/A