Registration Dossier

Ecotoxicological information

Long-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
fish early-life stage toxicity
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1980
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The study is well documented and scientifically acceptable. The study was carried out according to old guidelines and under GLP.
Reason / purpose:
reference to same study
Qualifier:
according to
Guideline:
other: Recommended Bioassay Procedure for Egg and Fry stages of Fresh Water Fish
Deviations:
no
Qualifier:
according to
Guideline:
other: ASTM Standard Practice for Conducting Toxicity Tests on the Early Life Stages of Fishes
Deviations:
no
Principles of method if other than guideline:
The test was designed to find a maximum allowable toxicant concentration, i.e. that concentration that does not affect any of the life traits of the organism.
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
200 mL grab samples were taken for the analytical procedures
Vehicle:
yes
Details on test solutions:
test solutions were made in acetone
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
Test organisms were obtained from Spring Creek Trout Hatchery, Lewistown, Montana
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
60 d
Post exposure observation period:
no data
Hardness:
no data
Test temperature:
10 (+/-1.0) °C
pH:
7.7 - 8.3
Dissolved oxygen:
4.4 - 9.6
Salinity:
no data
Nominal and measured concentrations:
Measured concentrations: 0.0065, 0.015, 0.023, 0.056, 0.110 mg/L
Details on test conditions:
no data
Reference substance (positive control):
no
Duration:
60 d
Dose descriptor:
NOEC
Effect conc.:
> 0.11 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
other: none of the evaluated endpoints was affected by the compound
Details on results:
Endpoints studied: Hatchability of eggs of the rainbow trout, Percentage survival of the fry, growth and growht rate of the fry (both as length and as weight), morphology and behavioural aspects.
Results with reference substance (positive control):
not applicable
Reported statistics and error estimates:
no data
Validity criteria fulfilled:
yes
Conclusions:
In an early life stage test over a period of 60 days rainbow trout was not affected by DBPP up to a concentration of 0.11 mg/L.
Executive summary:

An early life stage test was carried out with the aim of establishing a Maximum Acceptable Toxicant Concentration (MATC) for rainbow trout exposed to DBPP. The MATC is considered to be the concentration that an organism can be exposed to without any negative effects. The endpoints studied were: Hatchability of eggs of the rainbow trout, Percentage survival of the fry, growth and growth rate of the fry (both as length and as weight), morphology and behavioural aspects.

It showed that up to the maximum concentration of 0.11 mg/L none of the endpoints were affected by DBPP. Therefore, it is concluded that the MATC > 0.11 mg/L.

Description of key information

One, well performed study (K2) was carried out with Rainbow trout. In this ELS-test the fish were exposed for 60 days with the aim to find a Maximum Allowable Tolerance Concentration, based on the evaluation of hatchability of eggs, percentage survival of the fry, growth and growth rate of the fry (both as length and as weight), morphology and behavioural aspects (Boudreau and McAllister, 1980). Based on this study it could be concluded that the NOEC was above 0.11 mg/L.

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater fish:
0.11 mg/L

Additional information

The Early Life Stage test (60-day) (Boudreau and McAllister, 1980) evaluated different endpoints (hatchability of the eggs, percentage survival of the fry, growth and growth rate of the fry, morphology and behavioural aspects). In this test a Maximum Tolerance Concentration of 0.11 mg/L was established, indicative of the fact that no effects were observed for any of the endpoints at any of the concentrations tested. Therefore, it could be concluded that the NOEC for this test was above 0.11 mg/L.