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EC number: 907-672-2
CAS number: -
The study was conducted in accordance with the Council Regulation (EC)
No 440/2008, Method C.4-D “Manometric Respirometry Test“(2008) and
OECD301F (adopted July 1992).
A suspension of Dibutyl phenyl phosphate in a mineral medium was
inoculated and incubated for 28 d under aerobic conditions. During this
period, degradation was followed by continuous automated BOD
Dibutyl phenyl phosphate showed:
- 0.0 % degradation after 7 days
- 19.7 % degradation after 14 days
- 52.0 % degradation after 21 days
- 60.9 % degradation after 28 days
Therefore, Dibutyl phenyl phosphate is considered to be “Readily
Biodegradable without reaching the ten-day-window“.
The reference compound sodium benzoate showed 85.1 % degradation after
Ready biodegradability was assessed based on Biochemical Oxygen Demand
(BOD) and measurement of the residual test substance amounts. The
degradability based on the BOD measurement was ca. 11%, while that based
on residual test substance amount was ca. 9%.
The degradability of each component of the test substance was calculated
by each peak on the LC/MS chromatograms for measurement of the residual
test substance amount. Degradability of tri-butyl phosphate ( TBP) was
0%, that of dibutyl phenyl phosphate (DBPP) was 0 -4% and that of butyl
diphenyl phosphate (BDPP) was over 98%.
All of TBP and DBPP remained. All of BDPP disappeared.
From these results, it was concluded that the test substance is not
readily biodegradable and that no transformation products were generated
under the conditions of this test.
It is suggested that the degradation pathway for the phosphate esters
most likely involves a stepwise enzymatic hydrolysis to orthophosphate
and the phenolic or alcohol moieties according to Barret et al (1969).
The alcohol and phenol would then be expected to undergo further
The current study describes a CO2 evolution/biodegradability test of
different phosphate esters, among which DBPP. Ultimate biodegradation
(expressed as complete conversion to CO2, H2O inorganic salts and normal
cellular products of bacteria) and primary biodegradation (expressed as
disappearance of the original material due to bacterial action) were
measured, however this summary describes only the test results of the
Summary on biodegradation in water: screening test is provided on the
overarching level "biodegradation" (section 4.1.3 of the CSR).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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