Reaction mass of aluminium and magnesium oxide and spinel (Mg(AlO2)2)

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo mammalian germ cell study: cytogenicity / chromosome aberration

Remarks:

Type of genotoxicity: chromosome aberration

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Basic data given

Data source

Reference

Reference Type:

publication

Title:

Chromosome aberrations in mice induced by aluminium chloride

Author:

Manna, G. K. and Das, R. K.

Year:

1972

Bibliographic source:

the nucleus 15(3): 180-186

Materials and methods

Principles of method if other than guideline:

The present study was undertaken to find out the qualitative and quantitative effects, dose and aberration frequency relationship, origin and fate of the breaks for the treatment of AlCl3 in the chromosomes of bone marrow cells of mice.

GLP compliance:

not specified

Type of assay:

chromosome aberration assay

Test material

Test material information

Constituent 1

Reference substance name:

Aluminium chloride

EC Number:

231-208-1

EC Name:

Aluminium chloride

Cas Number:

7446-70-0

IUPAC Name:

aluminum trichloride

Details on test material:

- Name of test material (as cited in study report): Aluminium chloride
- Analytical purity: no data

Test animals

Species:

mouse

Strain:

other: Mus musculus

Sex:

not specified

Administration / exposure

Route of administration:

intraperitoneal

Duration of treatment / exposure:

1, 2, 4, 8, 12, 16, 20, 24, 48, 72 hours

Frequency of treatment:

single treatment

Post exposure period:

no data

Doses / concentrations

Remarks:

Doses / Concentrations:
0.01 M, 0.05 M, 0.1 M AlCl3
Basis:
nominal conc.

No. of animals per sex per dose:

4

Control animals:

yes, concurrent vehicle

Examinations

Tissues and cell types examined:

Bone marrow cells of specimens were fixed at different time points. The usual schedule of sodium citrate-acetic acid alcohol-air drying-Giemsa stain was followed for the preparations of bone marrow cells.

Results and discussion

Test results

Sex:

not specified

Genotoxicity:

positive

Toxicity:

not specified

Vehicle controls validity:

valid

Negative controls validity:

not specified

Positive controls validity:

not specified

Any other information on results incl. tables

Control series: An examination of 2000 metaphase complements revealed 12 (0.6%) gap, 14 (0.7%) constriction and 2 (0.1%) chromatid breaks only.

Treated series: The types of chromosome aberrations at different concentrations and intervals were more or less similar. The effects could broadly be put into two main categories, on general morphology and on the individual chromosome.

The effects on the individual chromosome were manifested as gap, constriction, subchromatid and chromatid breaks and exchange type ones. Very likely all of them were isochromatid breaks because the same chromosome sometimes contained another chromatid break. Tissue fixed at one hour after the treatment had also a good number of chromosomal aberrations. The effect of AlCl3 on bone marrow chromosomes of mice was, therefore, non-delayed type and it continued without much change even at 72 hours. The values of the aberration frequency were not proportional to the different molar solutions used. However, the use of higher concentration of AlCl3 increased the aberration frequency to some extent.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information): positive
The effect in the control series was negligible as determined from 2000 metaphase complements. In the treated series, qualitatively the effect was more or less the same at different intervals as well as at different concentrations, in the form of erosion, stickiness, etc. as general and sub-chromatid, chromatid and chromosome breaks, translocations, gaps and constrictions in the individual chromosomes.
Higher concentration induced higher frequency but the increase was not proportional.