Reaction mass of aluminium and magnesium oxide and spinel (Mg(AlO2)2)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

dioxo-2,4-dioxa-1,5-dialumina-3-magnesapentane oxomagnesium alumane

EC Number:

701-289-2

Molecular formula:

Al, MgO, Mg(AlO2)2

IUPAC Name:

dioxo-2,4-dioxa-1,5-dialumina-3-magnesapentane oxomagnesium alumane

Details on test material:

Content: aluminium 708,000 mg/kg, magnesium 68,000 mg/kg, ammonium 22,000 mg/kg, and other ingredients
Batch number: Delivery of 21.04.2009
Expiry date: 2011-08-21

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Examined at 0 and 48 hours.

Test solutions

Vehicle:

no

Details on test solutions:

Direct weighings were prepared to give the desired series of test concentrations. 21.9, 47.9, 105.6, 231.6 and 509.7 mg of the test item were added each to 1 litre of dilution water, treated for 1 h in an ultrasonic bath and stirred for 24 h on a magnetic stirrer. Undissolved particles of the test item were removed by filtration using folded filters of pore size 7-12 μm. The pH of each test solution was adjusted to 6.0.
19 mL of the solutions were taken and diluted with 1 mL of dilution water containing 10 daphnids resulting in final concentrations of 20.7, 45.5, 100, 220 and 484 mg/L. For each test item concentration and the control 2 replicates were prepared.

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20.0 to 21.0 °C

Nominal and measured concentrations:

Nominal: 20.7, 45.5, 100, 220 and 484 mg/L

Details on test conditions:

Experimental design : 5 test concentrations plus 1 control, 10 neonates per vessel, 2 replicates per concentration/control, no feeding during the exposure period
Photoperiod : 16 h light : 8 h dark

Reference substance (positive control):

not required

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 484 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 484 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The 48h-EC50 is expressed in term of actual loading of Aluminium-Skimmings.

The highest test concentration (484 mg/L nominally, corresponding to a mean measured concentration of 3.5 mg/L) exhibited a 20 % immobilisation effect after 24 h and 48 h of exposure.

Any other information on results incl. tables

Chemical analysis

Control	0h	48h
Aluminium determination (two replicates) [mg/L]	0.0082 / 0.0083	0.0017 / 0.0016
Aluminium determination (mean value) [mg/L]	0.0083	0.0017

The recoveries of Al at concentrations slightly higher than the Limit of Quantitation (1.0 μg/L) are not considered critical with respect to control performance.

100 mg/L	0h	48h
Aluminium determination (two replicates) [mg/L]	0.2027 / 0.2072	0.0862 / 0.0837
Aluminium determination (mean value) [mg/L]	0.2050	0.0850

220 mg/L	0h	48h
Aluminium determination (two replicates) [mg/L]	0.2161 / 0.2198	2.7922/ 2.8584
Aluminium determination (mean value) [mg/L]	0.218	2.8253

484 mg/L	0h	48h
Aluminium determination (two replicates) [mg/L]	3.4998/ 3.5048	0.1218 / 0.1271
Aluminium determination (mean value) [mg/L]	3.5023	0.1245

Mean recovery rate (100, 220, 484 mg/L) at 0 h in %        0.34

Mean recovery rate (100, 220, 484 mg/L) at 48 h in %       0.24

Applicant's summary and conclusion

Executive summary:

The acute toxicity of Aluminia Skimmings towards daphnids is characterized by an EC50(48h) of >484 mg/L, (Currenta, 2010).