Registration Dossier

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report date:
2009

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Reaction mass of aluminium and magnesium oxide and spinel (Mg(AlO2)2)
EC Number:
701-289-2
Molecular formula:
Al, MgO, Mg(AlO2)2
IUPAC Name:
Reaction mass of aluminium and magnesium oxide and spinel (Mg(AlO2)2)
Details on test material:
Content: aluminium 708,000 mg/kg, magnesium 68,000 mg/kg, ammonium 22,000 mg/kg, and other ingredients
Batch number: Delivery of 21.04.2009
Expiry date: 2011-08-21

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h

Test conditions

Nominal and measured concentrations:
Nominal: 10, 100 and 1000 mg/L
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
Aluminium-Skimmings showed 0.0 % respiration inhibition of activated sludge at the highest test item concentration of 1000 mg/L.
Results with reference substance (positive control):
3,5-Dichlorophenol: a concentration of 5 mg/L shows an inhibiton of 28.9%, 10 mg/L shows an inhibiton of 71.4%, 20 mg/L shows an inhibition of 80.0%

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
respiratory rates of the 2 controls differ less than 15 % from each other, The EC50 of the reference compound 3,5-Dichlorophenol is in the range 5 – 30 mg/L
Executive summary:

The toxicity of Aluminia skimmings to microorganisms (activated sludge) was tested according to OECD guideline 209 'Activated Sludge, Respiration Inhibition Test' (1984). Aluminium-Skimmings showed 0.0 % respiration inhibition of activated sludge at a test item concentration of 1000 mg/L, (Currenta, 2009).

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