Reaction mass of aluminium and magnesium oxide and spinel (Mg(AlO2)2)

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

dioxo-2,4-dioxa-1,5-dialumina-3-magnesapentane oxomagnesium alumane

EC Number:

701-289-2

Molecular formula:

Al, MgO, Mg(AlO2)2

IUPAC Name:

dioxo-2,4-dioxa-1,5-dialumina-3-magnesapentane oxomagnesium alumane

Details on test material:

Content: aluminium 708,000 mg/kg, magnesium 68,000 mg/kg, ammonium 22,000 mg/kg, and other ingredients
Batch number: Delivery of 21.04.2009
Expiry date: 2011-08-21

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

activated sludge

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test conditions

Nominal and measured concentrations:

Nominal: 10, 100 and 1000 mg/L

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

Aluminium-Skimmings showed 0.0 % respiration inhibition of activated sludge at the highest test item concentration of 1000 mg/L.

Results with reference substance (positive control):

3,5-Dichlorophenol: a concentration of 5 mg/L shows an inhibiton of 28.9%, 10 mg/L shows an inhibiton of 71.4%, 20 mg/L shows an inhibition of 80.0%

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

respiratory rates of the 2 controls differ less than 15 % from each other, The EC50 of the reference compound 3,5-Dichlorophenol is in the range 5 – 30 mg/L

Executive summary:

The toxicity of Aluminia skimmings to microorganisms (activated sludge) was tested according to OECD guideline 209 'Activated Sludge, Respiration Inhibition Test' (1984). Aluminium-Skimmings showed 0.0 % respiration inhibition of activated sludge at a test item concentration of 1000 mg/L, (Currenta, 2009).