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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report date:
2010

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Reaction mass of aluminium and magnesium oxide and spinel (Mg(AlO2)2)
EC Number:
701-289-2
Molecular formula:
Al, MgO, Mg(AlO2)2
IUPAC Name:
Reaction mass of aluminium and magnesium oxide and spinel (Mg(AlO2)2)
Details on test material:
Content: aluminium 708,000 mg/kg, magnesium 68,000 mg/kg, ammonium 22,000 mg/kg, and other ingredients
Batch number: Delivery of 21.04.2009
Expiry date: 2011-08-21

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Examined at 0 and 96 hours.

Test solutions

Vehicle:
no
Details on test solutions:
A direct weighing was prepared to give the desired test concentration. 500.5 mg of the test item were added to 5 litres of dilution water (adjusted to pH 6), treated for one hour in an ultrasonic bath and stirred for 24 h on a magnetic stirrer. Undissolved particles of the test item were removed by filtration using a folded filter of the pore size 7-12 μm. The pH was measured to be 7.0 and was re-adjusted to 6.0.
The concentration of the test item was determined analytically by ICP-MS. Finally 7 fish were given to the test item concentration and the control.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Date supplied : 2009-10-13
Acclimatisation : Stock held since 2009-10-13 and acclimatised to the test conditions since then.
Feeding : Commercial fish food, daily. Discontinued 24 h prior to test.
Mortalities during acclimatisation period : < 5 %
Medication : none

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Hardness:
dilution water: 239 mg/L CaCO3
Nominal and measured concentrations:
Nominal: 100 mg/L
Details on test conditions:
Experimental design : 1 test concentration plus 1 control, 7 fish per test concentration, no feeding during the exposure period
Reference substance (positive control):
not required

Results and discussion

Effect concentrationsopen allclose all
Duration:
2 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
The LC50 (2h – 96h) are expressed in terms of actual loadings of Aluminium-Skimmings.

Any other information on results incl. tables

Sublethal observations / clinical signs:

Chemical analysis

Control  0h  96h

 Aluminium determination (two replicates) [mg/L]

  < 0.01 /   < 0.01  < 0.01 / <0.01

 Aluminium determination (mean value) [mg/L]

   < 0.01  < 0.01

Limit of quantification = 0.01 mg/L

 100 mg/L  0h  96h

 Aluminium determination (two replicates) [mg/L]

 0.025 / 0.018  < 0.01 / <0.01

 Aluminium determination (mean value) [mg/L]

 0.022  < 0.01*

*value below the limit of quantification (0.01 mg/L); half the limit of quantification (0.005 mg/L) was used for further calculations.

Applicant's summary and conclusion

Executive summary:

A 96h-LC50 of >100 mg/l was determined with Danio rerio (Currenta, 2010).