Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Reason / purpose for cross-reference:
reference to same study
according to guideline
other: Range finding test for Study acc. to OECD 305
Principles of method if other than guideline:
Deviation from test plan: the amount of feed in the test plan was 2% of the fish weight, but it was actually 1.5%.
GLP compliance:
not specified
Range finding test for GLP Study acc. to OECD 305
Analytical monitoring:
not specified
not specified
Test organisms (species):
Oryzias latipes
Details on test organisms:
- Common name: Japanese rice fish
- Source: bred at testing facility
- Length at study initiation (length definition, mean, range and SD): 2 ± 1 cm
- Weight at study initiation (mean and range, SD): ca. 0.2 g

- Acclimation period: 6 wk
- Acclimation conditions (same as test or not): flow-through conditions
- Type and amount of food during acclimation: Tertramin fishfood (Tetra)
- Feeding frequency during acclimation: dayil, approx. 1.5 % of the body weight (no feeding 24 hours before the test)
- Health during acclimation (any mortality observed): <5% dead fish during 1 week before test (no medication of fish)

Test type:
Water media type:
Limit test:
Total exposure duration:
96 h
Test temperature:
24± 1 °C
Nominal and measured concentrations:
5 mg/L
Details on test conditions:
details on test system are not available

- Source/preparation of dilution water: Yokohama city tap water was dechlorinated by activated carbon filtration and sodium thiosulfate addition and used as test water.
Reference substance (positive control):
not specified
96 h
Dose descriptor:
Effect conc.:
> 5 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Validity criteria fulfilled:
not specified

Description of key information

Not acutely toxic to fish

Key value for chemical safety assessment

Additional information

The assessment of the acute toxicity to fish is based on the results of a range finding study performed in the framework of a bioaccumulation study following OECD 305. Fish of the species Oryzias latipes were exposed to a substance concentration of 5 mg/L in a 96 hour flow-through test. Mortalities were not observed. The determined LC50 (96 h) was >5 mg/L.