1,4,5,6,7,7-hexachloro-8,9,10-trinorborn-5-ene-2,3-dicarboxylic anhydride

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

No data

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study undertaken and reported scientifically but not to GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method B.1 bis (Acute Oral Toxicity - Fixed Dose Procedure)

Deviations:

not specified

GLP compliance:

no

Test type:

fixed dose procedure

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,4,5,6,7,7-hexachloro-8,9,10-trinorborn-5-ene-2,3-dicarboxylic anhydride

EC Number:

204-077-3

EC Name:

1,4,5,6,7,7-hexachloro-8,9,10-trinorborn-5-ene-2,3-dicarboxylic anhydride

Cas Number:

115-27-5

Molecular formula:

C9H2Cl6O3

IUPAC Name:

1,4,5,6,7,7-hexachloro-8,9,10-trinorborn-5-ene-2,3-dicarboxylic anhydride

Details on test material:

- Name of test material (as cited in study report): Chlorendic anhydride- Analytical purity: 98.81%- Lot/batch No.: 3-12-206

Test animals

Species:

rat

Strain:

other: Charles River CD

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS- Source: Charles River Breeding Laboratories, Inc., Portage, Michigan- Age at study initiation: - Weight at study initiation: 200 to 232 g- Fasting period before study: Overnight period immediately preceding oral administration during which food but not water was withheld- Housing: Hanging wire mesh cages.- Diet: Purina laboratory chow ad libitum- Water: ad libitum- Acclimation period:ENVIRONMENTAL CONDITIONS- Temperature (°C): - Humidity (%): - Air changes (per hr): - Photoperiod (hrs dark / hrs light): IN-LIFE DATES: From: To:

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Details on oral exposure:

VEHICLE- Concentration in vehicle: 807.1, 1281, 2034, 3229 and 5126 mg/kg- Amount of vehicle (if gavage): 10 ml/kg- Justification for choice of vehicle:- Lot/batch no. (if required): - Purity: MAXIMUM DOSE VOLUME APPLIED: DOSAGE PREPARATION (if unusual):CLASS METHOD (if applicable)- Rationale for the selection of the starting dose:

Doses:

807.1, 1281, 2034, 3229 and 5126 mg/kg

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days - Frequency of observations and weighing: Immediately before dosing, 7 days and 14 days.- Necropsy of survivors performed: yes- Other examinations performed: clinical signs, body weight,histopathology

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

2 562 mg/kg bw

95% CL:

2 218 - 2 961

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

2 130 mg/kg bw

95% CL:

1 698 - 2 671

Effect levels 3

Sex:

male/female

Dose descriptor:

LD50

Effect level:

2 336 mg/kg bw

95% CL:

2 065 - 2 643

Mortality:

2034 mg/kg - 2 female3229 mg/kg - 5 male; 5 female5126 mg/kg - 5 male; 5 female

Clinical signs:

other: At all dose rates, diarrhoea was evident. At 2034 mg/kg and above, hypoactivity, ataxia and alopecia were noted.

Applicant's summary and conclusion

Interpretation of results:

relatively harmless

Remarks:

Migrated informationCriteria used for interpretation of results: EU

Conclusions:

As the LD50 > 2000 mg/kg for both sexes this substance is not classified as harmful.