1,4,5,6,7,7-hexachloro-8,9,10-trinorborn-5-ene-2,3-dicarboxylic anhydride

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 14 June 2016 to 24 June 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Remarks:

Monitoring of the concentration in chlorendic acid

Details on sampling:

no data

Vehicle:

no

Details on test solutions:

none

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish
- Strain: HAMILTON-BUCHANAN
- Source: Umweltbundesamt (UBA)
- Age at study initiation (mean and range, SD): sexually immature young fish
- Length at study initiation (length definition, mean, range and SD): 2 +/- 1 cm

FEEDING DURING TEST
- Food type: warmwater fishfood and daphnia
- Amount: about 1-2% of body weight per day
- Frequency: three times a day

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

1.03 mmol/L

Test temperature:

The temperature was in the range of 24.1 – 24.9 °C

pH:

The pH was in the range of 6.8 - 7.8 in the test media, 7.8 - 8.0 in the blank control

Dissolved oxygen:

The dissolved oxygen content was in the range of 7.6 - 8.7 mg/l in the test media, 8.0 - 8.9 in the blank control

Conductivity:

242 µS/cm at 25°C

Nominal and measured concentrations:

see additional information on materials and methods

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: maximal volume 10 L
- Aeration: accomplished with glass tubes
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: drinking water
- Total organic carbon: 0.50 mg/l
- Pesticides:
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 12/12 hours, using neon tubes
- Light intensity: 23 +/- 2 °C

TEST CONCENTRATIONS
- Test concentrations: Nominal: 0, 100mg/l

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: No abnormal behaviour of the surviving fish was observed.
- Mortality of control: none
- Other adverse effects control: none

Results with reference substance (positive control):

none

Validity criteria fulfilled:

yes

Conclusions:

The test material did not induce death of the selected test species Danio rerio with the LC50 being greater than 100 mg/L.

Executive summary:

The short-term toxicity of Chlorendic Anhydride to the fresh water fish species Danio rerio was examined in accordance to the OECD guideline for the testing of chemicals 203. As Chlorendic Anhydride forms Chlorendic Acid in aqueous medium, the degradation product was actually tested.

A 96 hours toxicity test was performed on the fresh water fish species Danio rerio. A limit test was performed at a concentration of 100 mg/l with analytical monitoring and the LC50 value was determined by observation of mortality. The tests were conducted under controlled conditions. Dosing was performed under static conditions. Negative controls was valid.

The test material did not induce death of the selected test species Danio rerio with the LC50 being greater than 100 mg/L.

Description of key information

 The short-term toxicity of Chlorendic Anhydride to the fresh water fish species Danio rerio was examined in accordance to the OECD guideline for the testing of chemicals 203. As Chlorendic Anhydride forms Chlorendic Acid in aqueous medium, the degradation product was actually tested. A 96 hours toxicity test was performed on the fresh water fish species Danio rerio. A limit test was performed at a concentration of 100 mg/l with analytical monitoring and the LC50 value was determined by observation of mortality. The tests were conducted under controlled conditions. Dosing was performed under static conditions. Negative controls was valid. The test material did not induce death of the selected test species Danio rerio with the LC50 being greater than 100 mg/L. 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L