2-mercaptoethanol

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Restrictions: No guideline study, no GLP study, low number of animals, post exposure observation period 7 days, LD50 not calculated

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1965

Report date:

1965

Materials and methods

Principles of method if other than guideline:

test method according to Hill, Ass. Food and Drug Off. 18, 1954

GLP compliance:

no

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

2-mercaptoethanol

EC Number:

200-464-6

EC Name:

2-mercaptoethanol

Cas Number:

60-24-2

Molecular formula:

C2H6OS

IUPAC Name:

2-sulfanylethan-1-ol

Details on test material:

purity 88-97%, up to 5% water, up to 2% thiodiglycol

Test animals

Species:

rabbit

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

Rabbits initial bw 2.26-2.5 kg

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

Application of 0.05, 0.1 or 0.2 ml undiluted TS per kg bw (corresponding to 56, 112, 224 mg/kg bw; density 1.12 g/ml, on the clipped back (area 40-70 cm²); occlusive; animals immobilized; exposure duration 24 h; post exposure observation period 7 d; 3 rabbits per dose; necropsy performed.

Duration of exposure:

24 h

Doses:

56, 112, 224 mg/kg (= 0.05, 0.1, 0.2 ml/kg)

No. of animals per sex per dose:

3

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 7 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Statistics:

none

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 112 - ca. 224 mg/kg bw

Mortality:

0.2 ml/kg (exposed area about 50 cm2): 2/2 (within 8 - 20 h)
0.1 ml/kg (exposed area about 48 - 76 cm2): 1/3 (within 8 - 20 h)
0.005 ml/kg (exposed area about 42 - 68 cm2): 0/3

Clinical signs:

other: 0.2 ml/kg bw: no specific resorptive intoxication symptoms observed; necropsy: one rabbit with moderate congestion of the lung. 0.1 ml/kg bw: apathy, local reddening and edema; necropsy of this rabbit: atelectase of the lung, congestion of liver and kidne

Gross pathology:

0.1 ml/kg: atelectase of the lung, congestion of liver and kidney.
0.05 ml/kg bw: no effects.

Any other information on results incl. tables

Mortality:

Dose	Exposed area	Mortality
0.2 ml/kg	ca. 50 cm2	3/3 (within 8 - 20 h)
0.1 ml/kg	ca. 48 - 76 cm2	1/3 (within 8 - 20 h)
0.05 ml/kg	ca. 42 - 68 cm2	0/3