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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Restrictions: No guideline study, no GLP study, low number of animals, post exposure observation period 7 days, LD50 not calculated

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1965
Report date:
1965

Materials and methods

Principles of method if other than guideline:
test method according to Hill, Ass. Food and Drug Off. 18, 1954
GLP compliance:
no
Test type:
standard acute method

Test material

Constituent 1
Chemical structure
Reference substance name:
2-mercaptoethanol
EC Number:
200-464-6
EC Name:
2-mercaptoethanol
Cas Number:
60-24-2
Molecular formula:
C2H6OS
IUPAC Name:
2-sulfanylethan-1-ol
Details on test material:
purity 88-97%, up to 5% water, up to 2% thiodiglycol

Test animals

Species:
rabbit
Strain:
not specified
Sex:
male/female
Details on test animals or test system and environmental conditions:
Rabbits initial bw 2.26-2.5 kg

Administration / exposure

Type of coverage:
occlusive
Vehicle:
unchanged (no vehicle)
Details on dermal exposure:
Application of 0.05, 0.1 or 0.2 ml undiluted TS per kg bw (corresponding to 56, 112, 224 mg/kg bw; density 1.12 g/ml, on the clipped back (area 40-70 cm²); occlusive; animals immobilized; exposure duration 24 h; post exposure observation period 7 d; 3 rabbits per dose; necropsy performed.
Duration of exposure:
24 h
Doses:
56, 112, 224 mg/kg (= 0.05, 0.1, 0.2 ml/kg)
No. of animals per sex per dose:
3
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 7 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs
Statistics:
none

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
ca. 112 - ca. 224 mg/kg bw
Mortality:
0.2 ml/kg (exposed area about 50 cm2): 2/2 (within 8 - 20 h)
0.1 ml/kg (exposed area about 48 - 76 cm2): 1/3 (within 8 - 20 h)
0.005 ml/kg (exposed area about 42 - 68 cm2): 0/3
Clinical signs:
other: 0.2 ml/kg bw: no specific resorptive intoxication symptoms observed; necropsy: one rabbit with moderate congestion of the lung. 0.1 ml/kg bw: apathy, local reddening and edema; necropsy of this rabbit: atelectase of the lung, congestion of liver and kidne
Gross pathology:
0.1 ml/kg: atelectase of the lung, congestion of liver and kidney.
0.05 ml/kg bw: no effects.

Any other information on results incl. tables

Mortality:

 Dose  Exposed area  Mortality
 0.2 ml/kg  ca. 50 cm2  3/3 (within 8 - 20 h)
 0.1 ml/kg  ca. 48 - 76 cm2  1/3 (within 8 - 20 h)
 0.05 ml/kg  ca. 42 - 68 cm2  0/3

Applicant's summary and conclusion