Registration Dossier

Environmental fate & pathways

Additional information on environmental fate and behaviour

Administrative data

Endpoint:
additional information on environmental fate and behaviour
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable scientific method though mechanism of fate processes not clearly identified in the study report (Therefore, fate process drawn from expert judgement)

Data source

Reference
Reference Type:
publication
Title:
The stabilities of various thiol compounds used in protein purifications
Author:
Stevens R, Stevens L and Priof NC
Year:
1983
Bibliographic source:
Biochemical education 11(2), 70.

Materials and methods

Test guideline
Qualifier:
no guideline followed
GLP compliance:
not specified
Type of study / information:
Abiotic degradation of several thiols.

Test material

Reference
Name:
Unnamed
Type:
Constituent

Results and discussion

Any other information on results incl. tables


In summary, the half-live in hours (H) were influenced by the pH, temperature and metal ions (the by-products were not identified):

pH 6.5 20°C > 100 H
pH 7.5 20°C 10 H
pH 8.5 20°C 4 H

Temperature
pH 8.5 40°C 1 H
pH 8.5 20°C 4 H
pH 8.5 0°C 21 H

Metal ions: only Cu 2+ affects 2-ME stability (no effect of Fe3+, Cr3+, Ni2+, Fe2+, Mn2+, Zn2+, Ag+):
pH 8.5 20°C 4 H
pH 8.5 + 0.1 mM Cu2+ 20°C 0.6 H
pH 8.5 + 10 µM Cu2+ 20°C 1.5 H
pH 8.5 + 1.0 mM EDTA 20°C > 100 H
No significant effect recorded with 1.0 µM Cu2+.
Stability of TS increased by chelating agent EDTA.

Similar results with 0.01 M or 0.2 M potassium phosphate buffer or 0.1 M tris-HCl at the same pH.
Under the conditions used, loss of TS obeyed 1st-order kinetics.

Applicant's summary and conclusion