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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Additional information on environmental fate and behaviour

Administrative data

additional information on environmental fate and behaviour
Type of information:
experimental study
Adequacy of study:
key study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable scientific method though mechanism of fate processes not clearly identified in the study report (Therefore, fate process drawn from expert judgement)

Data source

Reference Type:
The stabilities of various thiol compounds used in protein purifications
Stevens R, Stevens L and Priof NC
Bibliographic source:
Biochemical education 11(2), 70.

Materials and methods

Test guideline
no guideline followed
GLP compliance:
not specified
Type of study / information:
Abiotic degradation of several thiols.

Test material

Constituent 1
Chemical structure
Reference substance name:
EC Number:
EC Name:
Cas Number:
Molecular formula:

Results and discussion

Any other information on results incl. tables

In summary, the half-live in hours (H) were influenced by the pH, temperature and metal ions (the by-products were not identified):

pH 6.5 20°C > 100 H
pH 7.5 20°C 10 H
pH 8.5 20°C 4 H

pH 8.5 40°C 1 H
pH 8.5 20°C 4 H
pH 8.5 0°C 21 H

Metal ions: only Cu 2+ affects 2-ME stability (no effect of Fe3+, Cr3+, Ni2+, Fe2+, Mn2+, Zn2+, Ag+):
pH 8.5 20°C 4 H
pH 8.5 + 0.1 mM Cu2+ 20°C 0.6 H
pH 8.5 + 10 µM Cu2+ 20°C 1.5 H
pH 8.5 + 1.0 mM EDTA 20°C > 100 H
No significant effect recorded with 1.0 µM Cu2+.
Stability of TS increased by chelating agent EDTA.

Similar results with 0.01 M or 0.2 M potassium phosphate buffer or 0.1 M tris-HCl at the same pH.
Under the conditions used, loss of TS obeyed 1st-order kinetics.

Applicant's summary and conclusion