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Acute test on all three trophic levels were performed to examine the aquatic toxicity of 2 -mercaptoethanol. Daphnia magna turned out to be the most sensitive species revealing an EC50 (48 h) of 0.4 mg/L. Fish and alga turned out to be less sensitive providing a LC50 (96 h) and ErC50 (72 h) of 37 mg/L and 19 mg/L respectively. Thus, 2-mercaptoethanol is considered acutely very toxic for aquatic organisms. Concerning chronic data on aquatic toxicity, a NOEC of 1.7 mg/L was found for algae (growth rate) while the NOEC for daphnids was measured to be 0.0632 mg/L. Thus, 2 -mercaptoethanol is considered chronically very toxic for aquatic organisms.