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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
comparable to guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report Date:
1990

Materials and methods

Test guidelineopen allclose all
Qualifier:
equivalent or similar to
Guideline:
EPA OTS 797.1400 (Fish Acute Toxicity Test)
Qualifier:
according to
Guideline:
other: Bulletin of National Affairs, Inc. Environmental Effects. Section 797-1400. Fish acute toxicity test. 12 July 1987. 31:6201.
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
-Purity: 99.8%

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Nominal and measured concentrations:
Nominal: 0, 6.25, 12.5, 25, 50, 100 and 200 mg/L
Measured: 0, 6.8, 13, 28, 51, 98, 210 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type: Open
- Material, size, headspace, fill volume: exposure chambers: 20.5 (length) x 21 (width) x 26 (height) cm; 18 cm depth.
- Aeration: No
- Renewal rate of test solution (frequency/flow rate): Volume of each replicate was exchanged seven times daily.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
42.9 mg/L
Nominal / measured:
meas. (not specified)
Basis for effect:
mortality
Remarks on result:
other: 35.4-52.5 mg/L

Any other information on results incl. tables

Cumulative Mortality in Percent

Mean Measured Concentration (mg/L)

24 Hours

48 Hours

72 Hours

96 Hours

A

B

A

B

A

B

A

B

Control

0

0

0

0

0

0

0

0

6.8

0

0

0

0

0

0

0

0

13

0

0

0

0

0

0

0

10

28

0

0

0

10

0

10

10

10

51

10

10

30

40

40

80

40

80

98

100

100

100

100

100

100

100

100

210

100

100

100

100

100

100

100

100

Applicant's summary and conclusion

Conclusions:
This study established a 96 hour LC50 for o-phenylenediamine in rainbow trout fingerlings of 42.9 mg/L.
Executive summary:

oPDA was tested in a 96-hour, flow-through test using fingerling rainbow trout with measured concentrations of 6.8, 13, 28, 51, 98, and 210 mg/L. All chemical and physical parameters were within expected ranges. The 96-hour LC50 was 42.9 mg/L.