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Ecotoxicological information

Toxicity to terrestrial plants

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Administrative data

Endpoint:
toxicity to terrestrial plants: short-term
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Induction of Somatic Mutations in Tradescantia clone 4430 by Three Phenylenediamine Isomers and the Antimutagenic Mechanisms of Diethyldithiocarbamate and Ammonium Meta-Vanadate
Author:
Gichner T, Cabrera Lopez G, Wagner ED, and Plewa MJ
Year:
1994
Bibliographic source:
Mutation Research, 306: 165-172

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Mutagenicity assay in plants was reported.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): o-phenylenediamine
- Physical state: Solid

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
yes

Test organisms

Species:
other: Tradescantia clone 4430
Details on test organisms:
a hybrid of T. subacaulis X T. hirsutiflora cultivated in a greenhouse at the Institute of Experimental Botany, Prague

Study design

Test type:
other: Mutagenicity assay
Study type:
laboratory study
Total exposure duration:
15 d

Test conditions

Test temperature:
18-22°C
Nominal and measured concentrations:
25, 50, 75, 100, 150, and 200 mM o-phenylenediamine dissolved in 0.2 M citrate-phosphate buffer, plus control (citrate-phosphate buffer only).

Results and discussion

Any other information on results incl. tables

A concentration dependent increase in pink stamen hair mutations was seen in the concentration range of 50-150 mM o-phenylenediamine.

o-Phenylenediamine was found to be toxic at concentrations above 150 mM, and repressed flowering.

At 150 mM, o-phenylenediamine induced approximately a 28-fold increase in the mutant frequency over the negative control.

Applicant's summary and conclusion

Conclusions:
Under the conditions employed in this study, o-phenylenediamine is mutagenic to Tradescantia clone 4430 relative to the negative control. The mutagenic potency of o-phenylenediamine in Tradescantia was 5.60 stamen hair mutants/µmole.
Executive summary:

A mutagenicity assay in plants was conducted. Under the conditions employed in this study, o-phenylenediamine is mutagenic to Tradescantia clone 4430 relative to the negative control. The mutagenic potency of o-phenylenediamine in Tradescantia was 5.60 stamen hair mutants/µmole.